Human/Mouse/Rat p53 Antibody Summary
Asp7-Asp393
Accession # P04637
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human p53 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Rat p53 by Western Blot Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha ). The mean integrated optical density is related to actin for Nrf2 (B), p53 (C) and mature 8-oxoguanine glycosylase alpha (D) levels. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05, **p < 0.01, ***p < 0.001 as compared to ST unless otherwise stated (horizontal line) for ANOVA with a Fisher post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26892894), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Product Datasheets
Citations for Human/Mouse/Rat p53 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats
Authors: Aiken CE, Tarry-Adkins JL, Spiroski AM et al.
FASEB J.
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Structure of the p53 degradation complex from HPV16
Authors: Wang, JCK;Baddock, HT;Mafi, A;Foe, IT;Bratkowski, M;Lin, TY;Jensvold, ZD;Preciado López, M;Stokoe, D;Eaton, D;Hao, Q;Nile, AH;
Nature communications
Species: Bacteria - Escherichia coli
Sample Types: Whole Cells
Applications: Ubiquitination -
Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
Oncogene, 2022-06-14;0(0):.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Simple Western -
Upregulation of hepatic autophagy under nutritional ketosis
Authors: D Li?kiewicz, A Li?kiewicz, M Grabowski, MM Nowacka-Ch, K Jab?o?ska, A Wojakowska, ? Marczak, JJ Barski, A Ma?ecki
The Journal of nutritional biochemistry, 2021-03-08;0(0):108620.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
CSN7B defines a variant COP9 signalosome complex with distinct function in DNA damage response
Authors: J Wang, D Dubiel, Y Wu, Y Cheng, DA Wolf, W Dubiel
Cell Reports, 2021-01-26;34(4):108662.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo
Redox Biol, 2016-12-18;11(0):219-230.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Long-term culture and expansion of primary human hepatocytes.
Authors: Levy G, Bomze D, Heinz S, Ramachandran S, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y
Nat Biotechnol, 2015-10-26;33(12):1264-1271.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
DISC-mediated activation of caspase-2 in DNA damage-induced apoptosis.
Authors: Olsson M, Vakifahmetoglu H, Abruzzo PM, Hogstrand K, Grandien A, Zhivotovsky B
Oncogene, 2009-04-06;28(18):1949-59.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage.
Authors: Vakifahmetoglu H, Olsson M, Orrenius S, Zhivotovsky B
Oncogene, 2006-05-01;25(41):5683-92.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats
Authors: Aiken CE, Tarry-Adkins JL, Spiroski AM et al.
J. Physiol. (Lond.)
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Long-term High Fat Ketogenic Diet Promotes Renal Tumor Growth in a Rat Model of Tuberous Sclerosis.
Authors: Liskiewicz AD, Kasprowska D, Wojakowska A et al.
Sci Rep
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