Human/Mouse/Rat p53 Agarose-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: p53

p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

Product Datasheets

Related Research Areas

• Apoptosis Transcription Factors and Regulators
• Cancer Biomarkers
• Cancer Stem Cell Transcription Factors
• Cell Cycle
• Checkpoint
• DNA Damage and Repair
• Epithelial Stem Cell Transcription Factors
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Immunoglobulin-Like Domain Transcription Factors
• Mesenchymal Stem Cell Transcription Factors
• Neural Stem Cell Transcription Factors
• p53 Pathway
• Parkinson's Disease Related Molecules
• Pluripotent Stem Cell Transcription Factors
• TNF Superfamily Regulators
• Transcription Factors and Regulators
• Transcription Factors in the Akt Pathway