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Human/Mouse/Rat p27/Kip1 Antibody

Catalog #: MAB2256
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2256
MAB2256-SP
Detection of Human/Mouse/Rat p27/Kip1 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p27/Kip1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p27 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 225501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat p27/Kip1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat p27/Kip1 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB2256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.

Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Entrez Gene IDs
1027 (Human); 12576 (Mouse); 83571 (Rat)
Alternate Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1

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Citations for Human/Mouse/Rat p27/Kip1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention.
    Authors: Prinz P, Mendler A, Brech D, Masouris I, Oberneder R, Noessner E
    Int J Cancer, 2014-03-26;135(8):1832-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. FOXO3a is involved in the apoptosis of naked oocytes and oocytes of primordial follicles from neonatal rat ovaries.
    Authors: Liu H, Luo LL, Qian YS, Fu YC, Sui XX, Geng YJ, Huang DN, Gao ST, Zhang RL
    Biochem. Biophys. Res. Commun., 2009-03-01;381(4):722-7.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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