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Human/Mouse/Rat Lipocalin-2/NGAL Antibody

Catalog #: AF1757
38 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
AF1757
AF1757-SP
Detection of Human Lipocalin‑2/NGAL by Western Blot.
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Product Details
Citations (38)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived Recombinant Human Lipocalin‑2/NGAL
Gln21-Gly198
Accession # P80188
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse and Rat Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Mouse and Rat Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of mouse and rat bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Lipocalin‑2/NGAL antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 34 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human Lipocalin-2/NGAL by Western Blot View Larger

Detection of Human Lipocalin-2/NGAL by Western Blot Enhanced expression of antimicrobial effectors by HO53 and HO56 stimulation in BCi cells. Samples were collected after 24 h (black bars) and 48 h (grey bars) post stimulation with Entinostat (10 μM), HO53 (75 μM) and HO56 (75 μM). Followed by expression analysis at mRNA and protein level by qRT-PCR and ELISA/Western blot, respectively. (a) Expression of LCN2 (lipocalin 2) at mRNA and (b) protein (lipocalin 2, NGAL) level in cell lysates. The representative Western blot is selected from one of the 3 independent experiments. GAPDH was used as a loading control. Full-length blots are presented in Supplementary Figure S10b. (c) Fold change of HBD1 (human beta -defensin 1) mRNA in comparison to control (Ctrl) and (d) secretion of hBD-1 peptide measured by ELISA in cell culture supernatants. (e) S100A8 expression was analyzed by qRT-PCR. TUBB (tubulin-beta ) was the reference gene in qRT-PCR. Each bar represents mean value of 3 independent experiments ± SEM; statistical significance was calculated in comparison to the control group using two-way ANOVA with Dunnett’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Significant changes are highlighted. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31068616), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry View Larger

Lipocalin‑2/NGAL in Human Pancreas Tissue Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # af1757) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells. Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.

Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Human/Mouse/Rat Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity
    Authors: Liu HH, Li XQ, Liu JF et al.
    Frontiers in Medicine
  2. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
    Authors: Kovacevic S, Ivanov M, Zivotic M Et al.
    Antioxidants (Basel)
  3. Expression of ACE2 in the Intact and Acutely Injured Kidney
    Authors: Nath K, Singh R, Grande J et al.
    Kidney360
  4. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma
    Authors: Asimakopoulou A, Vucur M, Luedde T et al.
    Cancers (Basel)
  5. Human conjunctiva organoids to study ocular surface homeostasis and disease
    Authors: Bannier-Hélaouët, M;Korving, J;Ma, Z;Begthel, H;Giladi, A;Lamers, MM;van de Wetering, WJ;Yawata, N;Yawata, M;LaPointe, VLS;Dickman, MM;Kalmann, R;Imhoff, SM;van Es, JH;López-Iglesias, C;Peters, PJ;Haagmans, BL;Wu, W;Clevers, H;
    Cell stem cell
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells, Whole Tissue
    Applications: IHC, Western Blot
  6. Lipocalin 2 activates the NLRP3 inflammasome via LPS?induced NF?kappaB signaling and plays a role as a pro?inflammatory regulator in murine macrophages
    Authors: SL Kim, MW Shin, SW Kim
    Molecular Medicine Reports, 2022-10-20;26(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. A timeline of oligodendrocyte death and proliferation following experimental subarachnoid hemorrhage
    Authors: Kang Peng, Sravanthi Koduri, Fenghui Ye, Jinting Yang, Richard F. Keep, Guohua Xi et al.
    CNS Neuroscience & Therapeutics
  8. Lipocalin 2 potentially contributes to tumorigenesis from colitis via IL-6/STAT3/NF-kB signaling pathway
    Authors: SL Kim, MW Shin, SY Seo, SW Kim
    Bioscience Reports, 2022-05-27;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  9. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
    Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
    International Journal of Molecular Sciences, 2022-02-10;23(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
    Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
    Nature Communications, 2021-12-17;12(1):7333.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  11. Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model
    Authors: AR Schiazza, EG Considine, M Betcher, BD Shepard
    Physiological Reports, 2021-12-01;9(23):e15007.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity
    Authors: Liu HH, Li XQ, Liu JF et al.
    Frontiers in Medicine
  13. Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors
    Authors: GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí
    International Journal of Molecular Sciences, 2021-08-10;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle
    Authors: J Brockhaus, RB Craveiro, I Azraq, C Niederau, SK Schröder, R Weiskirche, J Jankowski, M Wolf
    Biomolecules, 2021-06-23;11(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Exploring the human lacrimal gland using organoids and single-cell sequencing
    Authors: M Bannier-Hé, Y Post, J Korving, M Trani Bust, H Gehart, H Begthel, YE Bar-Ephrai, J van der Va, R Kalmann, SM Imhoff, H Clevers
    Cell Stem Cell, 2021-03-16;0(0):.
    Species: Human, Mouse
    Sample Types: Organoid
    Applications: IHC
  16. Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia
    Authors: B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha
    Cancers (Basel), 2020-07-31;12(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. TNF-&alpha controls Lipocalin-2 expression in PC-3 prostate cancer cells
    Authors: SK Schröder, A Asimakopou, S Tillmann, S Koschmiede, R Weiskirche
    Cytokine, 2020-07-24;135(0):155214.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Renal tubular cell spliced X-box binding protein 1 (Xbp1s) has a unique role in sepsis-induced acute kidney injury and inflammation
    Authors: Silvia Ferrè, Yingfeng Deng, Sarah C. Huen, Christopher Y. Lu, Philipp E. Scherer, Peter Igarashi et al.
    Kidney International
  19. Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking
    Authors: L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini
    Cell Rep, 2019-11-12;29(7):2067-2077.e6.
    Species: Human
    Sample Types: Cell Lysate
    Applications: IP
  20. Lipocalin‐2 Protects Against Diet‐Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes
    Authors: Yanyong Xu, Yingdong Zhu, Kavita Jadhav, Yuanyuan Li, Huihui Sun, Liya Yin et al.
    Hepatology Communications
  21. CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors
    Authors: M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa
    Cancer Med, 2019-05-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrity
    Authors: IT Myszor, Z Parveen, H Ottosson, P Bergman, B Agerberth, R Strömberg, GH Gudmundsso
    Sci Rep, 2019-05-08;9(1):7114.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Cardiac expression of neutrophil gelatinase-associated lipocalin in a model of cancer cachexia-induced cardiomyopathy
    Authors: Vincenzo Musolino, Sandra Palus, Celine Latouche, Micaela Gliozzi, Francesca Bosco, Federica Scarano et al.
    ESC Heart Failure
  24. Innovative in vitro method to study ventilator induced lung injury
    Authors: Jon P Joelsson, Iwona T Myszor, Ari J Arason, Saevar Ingthorsson, Paulina Cherek, Gabriel S Windels et al.
    ALTEX
  25. Lipocalin 2 inversely regulates TRAIL sensitivity through p38 MAPK-mediated DR5 regulation in colorectal cancer
    Authors: Se-Lim Kim, In Suk Min, Young Ran Park, Soo Teik Lee, Sang-Wook Kim
    International Journal of Oncology
  26. Glycine mitigates renal oxidative stress by suppressing Nox4 expression in rats with streptozotocin-induced diabetes
    Authors: Z Wang, J Zhang, L Wang, W Li, L Chen, J Li, D Zhao, H Zhang, X Guo
    J. Pharmacol. Sci., 2018-08-25;137(4):387-394.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection
    Authors: K Ramani, CV Jawale, AH Verma, BM Coleman, JK Kolls, PS Biswas
    JCI Insight, 2018-05-03;3(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Cilostazol attenuates kainic acid-induced hippocampal cell death
    Authors: YS Park, Z Jin, EA Jeong, CO Yi, JY Lee, IS Park, GS Roh
    Korean J. Physiol. Pharmacol., 2017-12-22;22(1):63-70.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  29. Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells
    Authors: YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko
    Oncotarget, 2017-11-06;8(62):105536-105552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares
    Authors: Shingo Haneda
    J. Reprod. Dev, 2016-12-16;0(0):.
    Species: Equine
    Sample Types: Luminal Fluid, Tissue Homogenates, Whole Tissue, Whole Tissue
    Applications: IHC-Fr, IHC-P, Western Blot
  31. The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
    Sci Rep, 2016-07-07;6(0):29243.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  32. Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion
    Authors: I-Hsiao Chung, Tzu-I Wu, Chia-Jung Liao, Jin-Yo Hu, Yang-Hsiang Lin, Pei-ju Tai et al.
    Oncotarget
  33. Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2
    Authors: Khalil El Karoui, Amandine Viau, Olivier Dellis, Alessia Bagattin, Clément Nguyen, William Baron et al.
    Nature Communications
  34. Activation of the unfolded protein response bypasses trastuzumab-mediated inhibition of the PI-3K pathway
    Authors: Sreekanth Kumandan, Navin R. Mahadevan, Kevin Chiu, Alexandra DeLaney, Maurizio Zanetti
    Cancer Letters
  35. Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.
    Authors: Leung, Lisa, Radulovich, Nikolina, Zhu, Chang-Qi, Organ, Shawna, Bandarchi, Bizhan, Pintilie, Melania, To, Christin, Panchal, Devang, Tsao, Ming Sou
    PLoS ONE, 2012-10-04;7(10):e46677.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  36. Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
    Authors: Wortmann A, He Y, Christensen ME, Linn M, Lumley JW, Pollock PM, Waterhouse NJ, Hooper JD
    J. Biol. Chem., 2011-10-12;286(49):42303-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Lipocalin 2 promotes breast cancer progression.
    Authors: Yang J, Bielenberg DR, Rodig SJ, Doiron R, Clifton MC, Kung AL, Strong RK, Zurakowski D, Moses MA
    Proc. Natl. Acad. Sci. U.S.A., 2009-02-23;106(10):3913-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  38. Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
    Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
    Kidney Int., 2008-10-01;75(3):285-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Human/Mouse/Rat Lipocalin-2/NGAL Antibody
By Peristera Ioanna Petropoulou on 02/16/2018
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human
Western Blot Human/Mouse/Rat Lipocalin-2/NGAL Antibody AF1757
Human/Mouse/Rat Lipocalin-2/NGAL Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human