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Human/Mouse/Rat JNK Pan Specific Antibody

Catalog #: AF1387
15 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
AF1387
AF1387-SP
Detection of Human/Mouse/Rat JNK by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat JNK Pan Specific Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human JNK1
Met1-Gln384
Accession # P45983
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
HEK293T human embryonic kidney cell line
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat JNK antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of recombinant JNK family members antibody by Western Blot. View Larger

Detection of recombinant JNK family members by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry JNK antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

JNK in Human Prostate Cancer Tissue. JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Immunohistochemistry JNK antibody in Rat Kidney by Immunohistochemistry (IHC-Fr). View Larger

JNK in Rat Kidney. JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western View Larger

Detection of Human JNK by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name
C-Jun N-terminal Kinase
Alternate Names
c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

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Citations for Human/Mouse/Rat JNK Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Pressure stress delays cyclooxygenase-2 expression induced by interleukin-1? in cultured human pulmonary artery smooth muscle cells
    Authors: Hiraide, S;Machida, T;Takihana, S;Ohshita, M;Iizuka, K;
    Heliyon
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth
    Authors: Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz
    iScience
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
    Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
    iScience
  4. Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug
    Authors: Emilia Alors-Perez, Ricardo Blázquez-Encinas, Sonia Alcalá, Cristina Viyuela-García, Sergio Pedraza-Arevalo, Vicente Herrero-Aguayo et al.
    Journal of Experimental & Clinical Cancer Research
  5. Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids
    Authors: J Aguado, HK Chaggar, C Gómez-Incl, MR Shaker, HC Leeson, A Mackay-Sim, EJ Wolvetang
    Aging Cell, 2021-08-30;0(0):e13468.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer
    Authors: JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque
    EBioMedicine, 2020-01-03;51(0):102547.
    Species: Human
    Sample Types: Cell Cultue Supernates
    Applications: Western Blot
  8. Role of the MAPK/cJun NH2-Terminal Kinase signaling pathway in starvation-induced autophagy
    Authors: SA Barutcu, N Girnius, S Vernia, RJ Davis
    Autophagy, 2018-08-17;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. The cJUN NH2-terminal kinase (JNK) signaling pathway promotes genome stability and prevents tumor initiation
    Authors: Nomeda Girnius, Yvonne JK Edwards, David S Garlick, Roger J Davis
    eLife
  10. JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins
    Authors: N Girnius, RJ Davis
    Cell Rep, 2017-11-14;21(7):1910-1921.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells
    Authors: L Courtial, V Picco, R Grover, Y Cormerais, C Rottier, A Labbe, G Pagès, C Ferrier-Pa
    Sci Rep, 2017-04-04;7(0):45713.
    Species: Coral - Stylophora pistillata
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2014-12-08;17(1):57-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
    Authors: Lopez R, Demick KP, Mansfield JM, Paulnock DM
    J. Immunol., 2008-10-01;181(7):4908-17.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
    Authors: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S
    J. Interferon Cytokine Res., 2008-04-01;28(4):235-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat JNK Pan Specific Antibody

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Human/Mouse/Rat JNK Pan Specific Antibody
By Anonymous on 06/07/2019
Application: WB Sample Tested: Lung single-cell suspension Species: Mouse
Western Blot Human/Mouse/Rat JNK Pan Specific Antibody AF1387
Human/Mouse/Rat JNK Pan Specific Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Western Blot Human/Mouse/Rat JNK Pan Specific Antibody AF1387