Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody Summary
Ser32-Asp296
Accession # P78504
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Jagged 1 in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 1
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).
- Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
- Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
- Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
- Lai, E.C. (2004) Development 131:965.
- Milner, L.A. et al. (1994) Blood 83:2057.
- Nyfeler, Y. et al. (2005) EMBO J. 24:3504
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Linheng, L. et al. (1998) Immunity 8:43.
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Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
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Oda, T. et al. (1997) Nat. Genet. 16:235.
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Oda, T. et al. (1997) Genomics 43:376.
Product Datasheets
Citation for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria
Authors: H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao
Int J Mol Sci, 2020-06-15;21(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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