Home / Jagged 1 / Human/Mouse/Rat Jagged 1 APC-conjugated Antibody

Human/Mouse/Rat Jagged 1 APC-conjugated Antibody

Catalog #: FAB1277A
1 Citation Datasheet
Catalog # Availability Size / Price Qty
FAB1277A
Detection of Jagged 1 in K562 Human Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Jagged 1 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry. 
Source
Monoclonal Mouse IgG2B Clone # 188331
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Jagged 1 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Jagged 1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC-conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Jagged 1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC-conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Product Datasheets

You must select a language.

x
Product Datasheet

Citation for Human/Mouse/Rat Jagged 1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance
    Authors: M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont
    Haematologica, 2019-10-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Jagged 1 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Jagged 1 APC-conjugated Antibody and earn rewards!

Have you used Human/Mouse/Rat Jagged 1 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review