Human/Mouse/Rat Jagged 1 Antibody Summary
Ser32-Asp296
Accession # P78504
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endo-thelioma cell line was stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
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Detection of Jagged 1 in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).
- Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
- Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
- Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
- Lai, E.C. (2004) Development 131:965.
- Milner, L.A. et al. (1994) Blood 83:2057.
- Nyfeler, Y. et al. (2005) EMBO J. 24:3504
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Linheng, L. et al. (1998) Immunity 8:43.
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Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
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Oda, T. et al. (1997) Nat. Genet. 16:235.
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Oda, T. et al. (1997) Genomics 43:376.
Product Datasheets
Citations for Human/Mouse/Rat Jagged 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Diabetes impairs arterio-venous specification in engineered vascular tissues in a perivascular cell recruitment-dependent manner
Authors: Wafa Altalhi
Biomaterials, 2016-12-08;119(0):23-32.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Jagged1 Instructs Macrophage Differentiation in Leprosy
PLoS Pathog, 2016-08-17;12(8):e1005808.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The CD46-Jagged1 interaction is critical for human TH1 immunity.
Authors: Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C
Nat Immunol, 2012-10-21;13(12):1213-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Authors: Luty WH, Rodeberg D, Parness J, Vyas YM
J. Immunol., 2007-07-15;179(2):819-29.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence.
Authors: Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M
Int. Immunol., 2006-10-11;18(12):1637-46.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Renewal of embryonic and neonatal-derived cardiac-resident macrophages in response to environmental cues abrogated their potential to promote cardiomyocyte proliferation via Jagged-1–Notch1
Authors: Rong Chen, Shiqing Zhang, Fang Liu, Lin Xia, Chong Wang, Siamak Sandoghchian Shotorbani et al.
Acta Pharmaceutica Sinica B
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