Human/Mouse/Rat IRS1 Antibody

Catalog #: AF3978 Datasheet

Catalog #	Availability	Size / Price	Qty
AF3978
AF3978-SP

Detection of Human, Mouse, and Rat IRS1 by Western Blot.

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Human/Mouse/Rat IRS1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat IRS1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IRS1
Ala801-Ala1020
Accession # P35568

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat IRS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IRS1 at approximately 170 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunohistochemistry

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IRS1 in Human Liver Cancer Tissue. IRS1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS1

IRS1 (Insulin Receptor Substrate 1) is a cytoplasmic protein that participates in the insulin signaling pathway. It is phosphorylated by the insulin receptor upon binding of insulin and subsequently associates with several SH2-domain-containing proteins.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1