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Human/Mouse/Rat HSP60 Antibody

Catalog #: MAB1800
7 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB1800
MAB1800-SP
Detection of Human/Mouse/Rat HSP60 by Western Blot.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSP60 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat HSP60.
Source
Monoclonal Mouse IgG2B Clone # 264233
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HSP60
Met1-Phe573
Accession # P10809
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat HSP60 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, DA3 mouse myeloma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry HSP60 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

HSP60 in HeLa Human Cell Line. HSP60 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western View Larger

Detection of Human, Mouse, and Rat HSP60 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 60 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human HSP60 by Western Blot View Larger

Detection of Human HSP60 by Western Blot Hsp60 induction and release after MPP+ treatment. (A) Hsp60 immunoblot analysis and quantification in cell lysates from SH-SY5Y after treatment with 100 μM MPP+ (at 6, 12, 24 and 48 hours) compared to control cells. (B) Hsp60 released by cultured SH-SY5Y cells treated by MPP+ (100 μM, at 6, 12, 24 and 48 hours) compared to control cultures. (C) MTT and lactate dehydrogenase (LDH) assay in SH-SY5Y cells exposed to MPP+ (100 μM, at 6, 12, 24 and 48 hours) compared to control cells. MTT assay (black bars), LDH assay (grey bars). *P < 0.05 versus corresponding controls. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24886419), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP60

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically upregulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress. Full length human HSP60 is 98% identical to mouse and rat HSP60.

Long Name
Heat Shock Protein 60
Entrez Gene IDs
3329 (Human); 110907 (Mouse); 63868 (Rat)
Alternate Names
60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13

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Citations for Human/Mouse/Rat HSP60 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Mitochondrial injury after mechanical stretch of cortical neurons in vitro: biomarkers of apoptosis and selective peroxidation of anionic phospholipids
    Authors: Jing Ji, Yulia Y. Tyurina, Minke Tang, Weihong Feng, Donna B. Stolz, Robert S.B. Clark et al.
    Journal of Neurotrauma
  2. Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells
    Authors: KT Stein, SJ Moon, AN Nguyen, HD Sikes
    PLoS Comput. Biol., 2020-09-14;16(9):e1008202.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo
    Authors: SY Choi, R Lopez-Gonz, G Krishnan, HL Phillips, AN Li, WW Seeley, WD Yao, S Almeida, FB Gao
    Nat. Neurosci., 2019-05-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. MICU1 Confers Protection from MCU-Dependent Manganese Toxicity
    Authors: J Wettmarsha, V Goh, KT Huang, DM Arduino, U Tripathi, A Leimpek, Y Cheng, AA Pittis, T Gabaldón, D Mokranjac, G Hajnóczky, F Perocchi
    Cell Rep, 2018-11-06;25(6):1425-1435.e7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
    Authors: Guangxin Li
    Am J Transl Res, 2016-10-15;8(10):4184-4194.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  6. Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.
    Authors: Noelker C, Morel L, Osterloh A, Alvarez-Fischer D, Lescot T, Breloer M, Gold M, Oertel W, Henze C, Michel P, Dodel R, Lu L, Hirsch E, Hunot S, Hartmann A
    J Neuroinflammation, 2014-05-08;11(0):86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP.
    Authors: Gotoh T, Oyadomari S, Mori K, Mori M
    J. Biol. Chem., 2002-01-22;277(14):12343-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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