Home / Glutathione Peroxidase 4/GPX4 / Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Catalog #: MAB5457
23 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB5457
MAB5457-SP
Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot.
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Citations (23)
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Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat GPX4 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 565320
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Glutathione Peroxidase 4
Gly74-Phe168
Accession # P36969
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Glutathione Peroxidase 4 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot. Western blot shows lysates of mouse, human, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 4 Monoclonal Antibody (Catalog # MAB5457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 4 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 4/GPX4

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21 kDa member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is 197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1‑27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1‑28. Over amino acids 74‑168, human GPX4 shares 93% aa identity with mouse GPX4.

Entrez Gene IDs
2879 (Human); 625249 (Mouse); 29328 (Rat)
Alternate Names
EC 1.11.1; EC 1.11.1.12; glutathione peroxidase 4 (phospholipid hydroperoxidase); Glutathione Peroxidase 4; GPX4; GPx-4; GSHPx-4; MCSP; PHGPx; PHGPxsnGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; snGPx; snPHGPx; sperm nucleus glutathione peroxidase

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Citations for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers
    Authors: Lei, G;Mao, C;Horbath, AD;Yan, Y;Cai, S;Yao, J;Jiang, Y;Sun, M;Liu, X;Cheng, J;Xu, Z;Lee, H;Li, Q;Lu, Z;Zhuang, L;Chen, MK;Alapati, A;Yap, TA;Hung, MC;You, MJ;Piwnica-Worms, H;Gan, B;
    Cancer discovery
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Oxidative stress induces mitochondrial iron overload and ferroptotic cell death
    Authors: Chen, Y;Guo, X;Zeng, Y;Mo, X;Hong, S;He, H;Li, J;Fatima, S;Liu, Q;
    Scientific reports
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
    Authors: Kose, T;Moreno-Fernandez, J;Vera-Aviles, M;Sharp, PA;Latunde-Dada, GO;
    Biochemistry and biophysics reports
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Mitochondrial defects leading to arrested spermatogenesis and ferroptosis in the PARL deficient mouse model of Leigh Syndrome
    Authors: Enrico Radaelli, Charles-Antoine Assenmacher, Jillian Verrelle, Esha Banerjee, Florence Manero, Salim Khiati et al.
    eLife
  5. Reply to: DHODH inhibitors sensitize to ferroptosis by FSP1 inhibition
    Authors: Mao, C;Liu, X;Yan, Y;Olszewski, K;Gan, B;
    Nature
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice
    Authors: J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani
    Science Advances, 2022-12-23;8(51):eadc9245.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  7. Upregulation of Nrf2 Signalling and the Inhibition of Erastin-Induced Ferroptosis by Ferulic Acid in MIN6 Cells
    Authors: T Kose, PA Sharp, GO Latunde-Da
    International Journal of Molecular Sciences, 2022-12-14;23(24):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis
    Authors: Zelong Gao, Dongyao Wang, Hongwei Zhang, Jianxin Yang, Min Li, Hongtao Lu et al.
    Current Research in Food Science
  9. Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing
    Authors: LB Pontel, A Bueno-Cost, AE Morellato, J Carvalho S, G Roué, M Esteller
    Redox Biology, 2022-07-31;55(0):102408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Erythroid lineage Jak2V617F expression promotes atherosclerosis through erythrophagocytosis and macrophage ferroptosis
    Authors: Wenli Liu, Nataliya Östberg, Mustafa Yalcinkaya, Huijuan Dou, Kaori Endo-Umeda, Yang Tang et al.
    Journal of Clinical Investigation
  11. A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria
    Authors: Shiqi Wu, Chao Mao, Lavanya Kondiparthi, Masha V. Poyurovsky, Kellen Olszewski, Boyi Gan
    Proceedings of the National Academy of Sciences
  12. A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers
    Authors: P Koppula, G Lei, Y Zhang, Y Yan, C Mao, L Kondiparth, J Shi, X Liu, A Horbath, M Das, W Li, MV Poyurovsky, K Olszewski, B Gan
    Nature Communications, 2022-04-22;13(1):2206.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
    Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
    Nature Communications, 2021-12-17;12(1):7333.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  14. Disrupting CISD2 function in cancer cells primarily impacts mitochondrial labile iron levels and triggers TXNIP expression
    Authors: Karmi O, Sohn YS, Zandalinas SI et al.
    Free Radical Biology and Medicine
  15. A Combined Drug Treatment That Reduces Mitochondrial Iron and Reactive Oxygen Levels Recovers Insulin Secretion in NAF-1-Deficient Pancreatic Cells
    Authors: Ola Karmi, Yang-Sung Sohn, Henri-Baptiste Marjault, Tal Israeli, Gil Leibowitz, Konstantinos Ioannidis et al.
    Antioxidants (Basel)
  16. DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer
    Authors: C Mao, X Liu, Y Zhang, G Lei, Y Yan, H Lee, P Koppula, S Wu, L Zhuang, B Fang, MV Poyurovsky, K Olszewski, B Gan
    Nature, 2021-05-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation
    Authors: Y Zhang, RV Swanda, L Nie, X Liu, C Wang, H Lee, G Lei, C Mao, P Koppula, W Cheng, J Zhang, Z Xiao, L Zhuang, B Fang, J Chen, SB Qian, B Gan
    Nature Communications, 2021-03-11;12(1):1589.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Energy-stress-mediated AMPK activation inhibits ferroptosis
    Authors: H Lee, F Zandkarimi, Y Zhang, JK Meena, J Kim, L Zhuang, S Tyagi, L Ma, TF Westbrook, GR Steinberg, D Nakada, BR Stockwell, B Gan
    Nat. Cell Biol., 2020-02-06;22(2):225-234.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. The role of ferroptosis in ionizing radiation-induced cell death and tumor suppression
    Authors: Guang Lei, Yilei Zhang, Pranavi Koppula, Xiaoguang Liu, Jie Zhang, Steven H. Lin et al.
    Cell Research
  20. Epigenetic regulation of AURKA by miR-4715-3p in upper gastrointestinal cancers
    Authors: A Gomaa, D Peng, Z Chen, M Soutto, K Abouelezz, A Corvalan, W El-Rifai
    Sci Rep, 2019-11-18;9(1):16970.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Reduced expression of the ferroptosis inhibitor glutathione peroxidase‐4 in multiple sclerosis and experimental autoimmune encephalomyelitis
    Authors: Che‐Lin Hu, Mara Nydes, Kara L. Shanley, Itzy E. Morales Morales Pantoja, Tamara A. Howard, Oscar A. Bizzozero
    Journal of Neurochemistry
  22. Targeting redox homeostasis in rhabdomyosarcoma cells: GSH-depleting agents enhance auranofin-induced cell death
    Authors: KJ Habermann, L Grünewald, S van Wijk, S Fulda
    Cell Death Dis, 2017-10-05;8(10):e3067.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
    Authors: Schoeneberger H, Belz K, Schenk B, Fulda S
    Oncogene, 2014-11-10;34(31):4032-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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