Human/Mouse/Rat FKBP12 Antibody Summary
Gly2-Glu108
Accession # P62942
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat FKBP12 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP12
FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. Proline isomerase activity was demonstrated but not used for quality control. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).
- Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119.
- Liu, J. et al. (1992) Biochemistry 31:3896.
- Toral-Barza, L. et al. (2005) Biochem. Biophys. Res. Comm. 332:304.
- Hamilton, S.L and M.M. Matzuk (1998) Nature 391:489.
Product Datasheets
Citations for Human/Mouse/Rat FKBP12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress
Authors: L Jiang, P Chakrabort, L Zhang, M Wong, SE Hill, CJ Webber, J Libera, LJ Blair, B Wolozin, M Zweckstett
Science Advances, 2023-02-01;9(5):eadd9789.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms
Authors: C Grohmann, CM Magtoto, JR Walker, NK Chua, A Gabrielyan, M Hall, SA Cobbold, S Mieruszyns, M Brzozowski, DS Simpson, H Dong, B Dorizzi, AV Jacobsen, E Morrish, N Silke, JM Murphy, JK Heath, A Testa, C Maniaci, A Ciulli, G Lessene, J Silke, R Feltham
Nature Communications, 2022-04-19;13(1):2073.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
An inducible, ligand-independent receptor activator of NF-kappaB gene to control osteoclast differentiation from monocytic precursors.
Authors: Rementer C, Wu M, Buranaphatthana W, Yang H, Scatena M, Giachelli C
PLoS ONE, 2013-12-27;8(12):e84465.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Designing Calcium Release Channel Inhibitors with Enhanced Electron Donor Properties: Stabilizing the Closed State of Ryanodine Receptor Type 1
Authors: Yanping Ye, Daniel Yaeger, Laura J. Owen, Jorge O. Escobedo, Jialu Wang, Jeffrey D. Singer et al.
Molecular Pharmacology
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