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Human/Mouse/Rat ER alpha /NR3A1 Antibody

Catalog #: MAB57151
4 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB57151
MAB57151-SP
Detection of Human, Mouse, and Rat ER alpha /NR3A1 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ER alpha /NR3A1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ER alpha /NR3A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ER beta is observed.
Source
Monoclonal Mouse IgG2B Clone # 681118
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ER alpha /NR3A1
Met1-Gln116
Accession # P03372
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat ERa/NR3A1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat ER alpha /NR3A1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.05 µg/mL of Human ERa/NR3A1 Monoclonal Antibody (Catalog # MAB57151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERa/NR3A1 at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human ERa/NR3A1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human ER alpha /NR3A1 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for ER alpha /NR3A1 at approximately 75 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat ER alpha /NR3A1 Monoclonal Antibody (Catalog # MAB57151). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER alpha/NR3A1

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins.  It is widely expressed, and serves as a strong activator of estrogen-responsive genes.  ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins.  Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators.  Human ER alpha is 595 amino acids (aa) in length.  It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575.  Major phosphorylation sites exist at Tyr537, Ser167 and Ser118.  Multiple splice forms exist.  There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595.  Human ER alpha is only 46% aa identical to human ER beta.  Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha.

Long Name
Estrogen Receptor alpha
Entrez Gene IDs
2099 (Human)
Alternate Names
ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1

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Citations for Human/Mouse/Rat ER alpha /NR3A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Abnormal expression of fibrosis markers, estrogen receptor alpha and stromal derived factor‑1/chemokine (C‑X‑C motif) receptor‑4 axis in intrauterine adhesions
    Authors: Qin Zhou, Xixi Wu, Jianguo Hu, Rui Yuan
    International Journal of Molecular Medicine
  2. Loss of epigenetic regulation disrupts lineage integrity, induces aberrant alveogenesis and promotes breast cancer
    Authors: E Langille, KN Al-Zahrani, Z Ma, M Liang, L Uuskula-Re, R Espin, K Teng, A Malik, H Bergholtz, S El Ghamras, S Afiuni-Zad, R Tsai, S Alvi, A Elia, Y Lu, RH Oh, KJ Kozma, D Trcka, M Narimatsu, JC Liu, T Nguyen, S Barutcu, SK Loganathan, R Bremner, GD Bader, SE Egan, DW Cescon, T Sorlie, JL Wrana, HW Jackson, MD Wilson, AK Witkiewicz, ES Knudsen, MA Pujana, GM Wahl, D Schramek
    Cancer Discovery, 2022-12-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. DNA methylation was involved in total glucosides of paeony regulating ERα for the treatment of female systemic lupus erythematosus mice
    Authors: M Li, A Jiang
    J. Pharmacol. Sci., 2019-07-10;0(0):.
    Species: Mouse
    Sample Types: Homogenized Tissues
    Applications: Western Blot
  4. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014-05-29;14(0):379.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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