Human/Mouse/Rat Cytochrome c Antibody

Catalog #: MAB899 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB899
MAB899-SP

Immunoprecipitation of Human/Mouse Cytochrome c.

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Human/Mouse/Rat Cytochrome c Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects native human, mouse, and rat Cytochrome c.

Source

Monoclonal Mouse IgG_2A Clone # 2.7D5

Purification

Protein A or G purified from ascites

Immunogen

Pigeon Cytochrome c

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immuno-precipitated from lysates (5 x 10⁶cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB899) for 1 hour at 4 °C. Cytochrome c-antibody comp-lexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecip-itated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Mouse Anti-Human/ Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytochrome c

Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1). Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1). Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3). Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3). Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4). After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.

References