Human/Mouse/Rat Cytochrome c Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Cytochrome c

Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1).  Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1).  Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3).  Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3).  Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4).  After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.

Product Datasheets

Related Research Areas

• ALS Related Molecules
• Bcl-2 Proteins and Regulators
• Mitochondrial Proteins