Human/Mouse/Rat Blk Antibody

Catalog #: AF2679 Datasheet

Catalog #	Availability	Size / Price	Qty
AF2679
AF2679-SP

Detection of Human/Mouse/Rat Blk by Western Blot.

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Human/Mouse/Rat Blk Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Blk in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Blk
Met1-Val63
Accession # P51451

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Blk by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Blk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Blk at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Blk

B-cell lymphocyte kinase (Blk) is a member of the Src family of non‑receptor tyrosine kinases. Constitutively active Blk induces malignant transformation of B-cell progenitors, suggesting a role for Blk in B-lineage cell differentiation and proliferation.

Long Name

B Lymphoid Tyrosine Kinase

Entrez Gene IDs

640 (Human); 12124 (Mouse); 364403 (Rat)

Alternate Names

B lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; Blk; EC 2.7.10.2; MGC10442; MODY11; p55-Blk; tyrosine-protein kinase Blk

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