Human/Mouse Meprin  beta  Subunit/MEP1B Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Meprin beta Subunit/MEP1B

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only) (3). Although the two subunits share 42% amino acid (aa) sequence identity, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of human Merpin beta subunit precursor consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 61), and a mature chain (aa 62 to 701) containing catalytic (aa 62 to 259), MAM (aa 260 to 429), MATH (aa 430 to 585), EGF-like (aa 604 to 644), transmembrane (aa 653 to 673), and cytoplasmic (aa 674 to 701) domains.

Product Datasheets

Related Research Areas

• Metalloproteases and Regulators
• Search Bioprocessing Enzymes