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Human/Mouse IGF-I R/IGF1R Antibody

Catalog #: AF-305-NA
24 Citations 3 Reviews Datasheet
Catalog # Availability Size / Price Qty
AF-305-NA
AF-305-SP
IGF-I R/IGF1R in MCF‑7 and HDLM-2 Human Cell Lines.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IGF-I R/IGF1R Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse IGF-I R/IGF1R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Recombinant Human IGF-I R/IGF1R extracellular domain
Accession # P08069
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGF‑I R/IGF1R (Catalog # 391-GR) and Recombinant Mouse IGF‑I R/IGF1R (Catalog # 6630-GR).
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
1-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGF-I-induced proliferation in the MCF-7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IGF-I R/IGF1R antibody in MCF-7 and HDLM-2 Human Cell Lines by Immunocytochemistry (ICC). View Larger

IGF-I R/IGF1R in MCF‑7 and HDLM-2 Human Cell Lines. IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and HDLM-2 human Hodgkin's lymphoma cell line (negative control, right panel) using Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslipsand Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IGF-I R/IGF1R antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

IGF-I R/IGF1R in Mouse Embryo. IGF-I R/IGF1R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry IGF-I R/IGF1R antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

IGF-I R/IGF1R in Human Placenta. IGF-I R/IGF1R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody. View Larger

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody. Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5-1.5 µg/mL.

Immunohistochemistry Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry View Larger

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry View Larger

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry View Larger

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry View Larger

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry View Larger

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References
  1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Citations for Human/Mouse IGF-I R/IGF1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development
    Authors: Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza et al.
    International Journal of Molecular Sciences
  2. Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3
    Authors: Niklas Ortenlöf, Suvi Vallius, Helena Karlsson, Claes Ekström, Amanda Kristiansson, Bo Holmqvist et al.
    Fluids and Barriers of the CNS
  3. Inhibition of the PI3K but not the MEK/ERK pathway sensitizes human glioma cells to alkylating drugs
    Authors: B Haas, V Klinger, C Keksel, V Bonigut, D Kiefer, J Caspers, J Walther, M Wos-Magang, S Weickhardt, G Röhn, M Timmer, R Frötschl, N Eckstein
    Cancer Cell Int., 2018-05-04;18(0):69.
  4. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS
    Sci Rep, 2016-05-16;6(0):25960.
  5. Inhibition of the insulin-like growth factor-1 receptor potentiates acute effects of castration in a rat model for prostate cancer growth in bone
    Authors: Annika Nordstrand, Sofia Halin Bergström, Elin Thysell, Erik Bovinder-Ylitalo, Ulf H. Lerner, Anders Widmark et al.
    Clinical & Experimental Metastasis
  6. IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy
    Authors: Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Inhibiting IGF1R-mediated Survival Signaling in Head and Neck Cancer with the Peptidomimetic SSTN(IGF1R)
    Authors: Stueven NA, Beauvais DM, Hu R et al.
    Cancer Research Communications
  8. Insulin-like Growth Factor Binding Protein 3 Increases Mouse Preimplantation Embryo Cleavage Rate by Activation of IGF1R and EGFR Independent of IGF1 Signalling
    Authors: CJ Green, M Span, MH Rayhanna, M Perera, ML Day
    Cells, 2022-11-24;11(23):.
    Species: Mouse
    Sample Types: Embryo
    Applications: Cell Culture
  9. CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway
    Authors: Y Wang, L Yin, X Sun
    J. Exp. Clin. Cancer Res., 2020-08-26;39(1):169.
    Species: Human
    Sample Types: Cell Lysate, Cell Lysates
    Applications: Western Blot
  10. IGF1R is an entry receptor for respiratory syncytial virus
    Authors: CD Griffiths, LM Bilawchuk, JE McDonough, KC Jamieson, F Elawar, Y Cen, W Duan, C Lin, H Song, JL Casanova, S Ogg, LD Jensen, B Thienpont, A Kumar, TC Hobman, D Proud, TJ Moraes, DJ Marchant
    Nature, 2020-06-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Determination of Mammalian Target of Rapamycin Hyperactivation as Prognostic Factor in Well-Differentiated Neuroendocrine Tumors
    Authors: G Lamberti, C Ceccarelli, N Brighi, I Maggio, D Santini, C Mosconi, C Ricci, G Biasco, D Campana
    Gastroenterol Res Pract, 2017-10-29;2017(0):7872519.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
    Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
    Cancer Res, 2017-03-10;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Functional Assay
  13. Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages
    Authors: L R Batista-Si
    Sci Rep, 2016-06-10;6(0):27632.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Luciferase Reporter Assay
  14. Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival.
    Authors: Ma J, Guo C, Guo C, Sun Y, Liao T, Beattie U, Lopez F, Chen D, Lashkari K
    PLoS ONE, 2015-04-29;10(4):e0125695.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation.
    Authors: Tworkoski K, Raab-Traub N
    J Virol, 2014-12-17;89(5):2590-602.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Neutralization
  16. IGF-IR mediated mammary tumorigenesis is enhanced during pubertal development.
    Authors: Jones R, Watson K, Campbell C, Moorehead R
    PLoS ONE, 2014-09-26;9(9):e108781.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.
    Authors: Mathur, Anjili, Ware, Christop, Davis, Lenora, Gazdar, Adi, Pan, Bo-Sheng, Lutterbach, Bart
    PLoS ONE, 2014-06-26;9(6):e98515.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
    Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
    Cancer Res., 2009-03-24;69(7):2996-3003.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas.
    Authors: Yu L, Saile K, Swartz CD, He H, Zheng X, Kissling GE, Di X, Lucas S, Robboy SJ, Dixon D
    Mol. Med., 2008-05-01;14(5):264-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. A possible function of Nik-related kinase in the labyrinth layer of delayed delivery mouse placentas
    Authors: Hiroshi YOMOGITA, Hikaru ITO, Kento HASHIMOTO, Akihiko KUDO, Toshiaki FUKUSHIMA, Tsutomu ENDO et al.
    Journal of Reproduction and Development
  21. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process
    Authors: Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al.
    Oncotarget
  22. Inhibition of the Insulin-Like Growth Factor-1 Receptor Enhances Effects of Simvastatin on Prostate Cancer Cells in Co-Culture with Bone
    Authors: Annika Nordstrand, Marie Lundholm, Andreas Larsson, Ulf H. Lerner, Anders Widmark, Pernilla Wikström
    Cancer Microenvironment
  23. Inhibiting IGF1R-mediated Survival Signaling in Head and Neck Cancer with the Peptidomimetic SSTN(IGF1R)
    Authors: Stueven NA, Beauvais DM, Hu R et al.
    Cancer Research Communications
  24. Insulin-like growth factor 1 acts as an autocrine factor to improve early embryogenesis in vitro
    Authors: Charmaine J. Green, Margot L. Day
    The International Journal of Developmental Biology

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Reviews for Human/Mouse IGF-I R/IGF1R Antibody

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Human/Mouse IGF-I R/IGF1R Antibody
By Anonymous on 12/18/2023
Application: WB
Western Blot Human/Mouse IGF-I R/IGF1R Antibody AF-305-NA
Human/Mouse IGF-I R/IGF1R Antibody
By Anonymous on 06/18/2021
Application: IHC Sample Tested: Cancer Tissue Species: Human
Immunohistochemistry Human/Mouse IGF-I R/IGF1R Antibody AF-305-NA
Human/Mouse IGF-I R/IGF1R Antibody
By Anonymous on 07/30/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Intestinal villi Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse IGF-I R/IGF1R Antibody AF-305-NA