Human/Mouse HO-1/HMOX1/HSP32 Antibody Summary
Met1-Thr261
Accession # P09601
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human/Mouse HO‑1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
View Larger
Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, an inducible Heme Oxygenase-1 (HO-1/HMOX1) and a constitutive Heme Oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.
Product Datasheets
Citations for Human/Mouse HO-1/HMOX1/HSP32 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Inner ear supporting cells protect hair cells by secreting HSP70
Authors: Lindsey A. May, Inga I. Kramarenko, Carlene S. Brandon, Christina Voelkel-Johnson, Soumen Roy, Kristy Truong et al.
Journal of Clinical Investigation
-
?2-AR inhibition enhances EGFR antibody efficacy hampering the oxidative stress response machinery
Authors: Del Vecchio, V;Mele, L;Panda, SK;Sanchez-Pajares, IR;Mosca, L;Tirino, V;Barbieri, M;Bruzzese, F;Luciano, A;Marino, FZ;Accardo, M;Nicoletti, GF;Papaccio, G;Barbieri, A;Desiderio, V;
Cell death & disease
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
Dysregulation of Nrf2 redox pathway in macrophages under diabetic microenvironment
Authors: GV Ganesh, KM Ramkumar
Experimental gerontology, 2021-07-10;152(0):111479.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mitochondrial alterations triggered by repeated exposure to fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) fractions of ambient particulate matter
Authors: J Sotty, J Kluza, C De Sousa, M Tardivel, S Anthérieu, LY Alleman, L Canivet, E Perdrix, A Loyens, P Marchetti, JM Lo Guidice, G Garçon
Environ Int, 2020-06-22;142(0):105830.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of heme oxygenase-1 in factor VIII-deficient mice reduces the immune response to therapeutic factor VIII.
Authors: Dimitrov JD, Dasgupta S, Navarrete AM, Delignat S, Repesse Y, Meslier Y, Planchais C, Teyssandier M, Motterlini R, Bayry J, Kaveri SV, Lacroix-Desmazes S
Blood, 2009-11-04;115(13):2682-5.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Heat Shock Protein-Mediated Protection Against Cisplatin-Induced Hair Cell Death
Authors: Tiffany G. Baker, Soumen Roy, Carlene S. Brandon, Inga K. Kramarenko, Shimon P. Francis, Mona Taleb et al.
Journal of the Association for Research in Otolaryngology
-
Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1
Authors: Olivia May, Nicolas S. Merle, Anne Grunenwald, Viviane Gnemmi, Juliette Leon, Cloé Payet et al.
Frontiers in Immunology
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse HO-1/HMOX1/HSP32 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse HO-1/HMOX1/HSP32 Antibody and earn rewards!
Have you used Human/Mouse HO-1/HMOX1/HSP32 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
