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Human/Mouse HIF-1 alpha/HIF1A Antibody

Catalog #: MAB1935
14 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB1935
MAB1935-SP
HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue.
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Citations (14)
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Human/Mouse HIF-1 alpha/HIF1A Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes human and mouse HIF-1 alpha/HIF1A in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 241812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIF-1 alpha/HIF1A
Arg575-Asn826
Accession # Q16665.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
CoCl2‑treated MCF‑7 human breast cancer cell line, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HIF-1 alpha/HIF1A antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue. HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse HIF-1 alpha/HIF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Clinical and prognostic significance of HIF-1alpha, PTEN, CD44v6, and survivin for gastric cancer: a meta-analysis.
    Authors: Chen J, Li T, Liu Q, Jiao H, Yang W, Liu X, Huo Z
    PLoS ONE, 2014-03-19;9(3):e91842.
  2. microRNA-142 guards against autoimmunity by�controlling�Treg�cell homeostasis and function
    Authors: WL Wang, C Ouyang, NM Graham, Y Zhang, K Cassady, EY Reyes, M Xiong, AM Davis, K Tang, D Zeng, MP Boldin
    PloS Biology, 2022-02-18;20(2):e3001552.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells
    Authors: Y Sun, X Lin, B Liu, Y Zhang, W Li, S Zhang, F He, H Tian, X Zhu, X Liu, J Wu, J Cai, M Li
    Science Advances, 2021-12-22;7(52):eabj6877.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tumor hypoxia represses &gamma&delta T cell-mediated antitumor immunity against brain tumors
    Authors: JH Park, HJ Kim, CW Kim, HC Kim, Y Jung, HS Lee, Y Lee, YS Ju, JE Oh, SH Park, JH Lee, SK Lee, HK Lee
    Nature Immunology, 2021-02-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Colorectal Cancer Cell Line SW480 and SW620 Released Extravascular Vesicles: Focus on Hypoxia-induced Surface Proteome Changes
    Authors: ILVA NAKURTE, KASPARS JEKABSONS, REINIS REMBERGS, ELINA ZANDBERGA, ARTURS ABOLS, AIJA LINĒ et al.
    Anticancer Research
  6. Extracellular Vesicles Derived from Hypoxic Colorectal Cancer Cells Confer Metastatic Phenotype to Non-metastatic Cancer Cells
    Authors: EDGARS ENDZELIŅŠ, ARTŪRS ĀBOLS, ARTŪRS BUŠS, ELĪNA ZANDBERGA, MARI PALVIAINEN, PIA SILJANDER et al.
    Anticancer Research
  7. Celastrol mediates Th17 and Treg cell generation via metabolic signaling
    Authors: J Zhang, J Shan, X Chen, S Li, D Long, Y Li
    Biochem. Biophys. Res. Commun., 2018-02-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
    Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
    Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
    Authors: Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson T, Rankin E, Giaccia A, Schipani E
    Dev Biol, 2014-06-24;393(1):124-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Expression of monocyte chemotactic protein-1/CCL2 in gastric cancer and its relationship with tumor hypoxia
    Authors: Lei-Lei Tao, Shu-Jing Shi, Long-Bang Chen, Gui-Chun Huang
    World Journal of Gastroenterology
  11. Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
    Authors: Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J
    Int J Clin Exp Pathol, 2014-01-15;7(2):575-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Expression and clinical significance of CD73 and hypoxia-inducible factor-1 alpha in gastric carcinoma
    Authors: Xiao-Xia Lu, Yi-Tian Chen, Bing Feng, Xiao-Bei Mao, Bo Yu, Xiao-Yuan Chu
    World Journal of Gastroenterology
  13. HBO1 Is Required for H3K14 Acetylation and Normal Transcriptional Activity during Embryonic Development
    Authors: Andrew J. Kueh, Mathew P. Dixon, Anne K. Voss, Tim Thomas
    Molecular and Cellular Biology
  14. Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
    Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S
    Cancer Res., 2007-06-15;67(12):5789-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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