Human/Mouse GSK-3 alpha / beta Antibody

Catalog #: MAB25063
2 Citations Datasheet

Catalog #	Availability	Size / Price	Qty
MAB25063
MAB25063-SP

Detection of Human GSK 3 alpha and GSK‑3 beta by Western Blot.

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All GSK-3 alpha/beta Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GSK-3 alpha / beta Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human GSK-3 beta in direct ELISAs and human GSK-3 alpha and GSK-3 beta in Western blots. Detects human and mouse GSK-3 alpha / beta in immunocytochemistry.

Source

Monoclonal Mouse IgG_2B Clone # 833916

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GSK-3 beta
Asp200-Phe305
Accession # P49841

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GSK 3 alpha and GSK‑3 beta by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GSK-3a at approximately 46 and GSK-3 beta at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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GSK‑3 alpha / beta in HT‑29 Human Cell Line. GSK-3a/ beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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GSK‑3 alpha / beta in NIH‑3T3 Mouse Cell Line. GSK-3a/ beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK-3 alpha/beta

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human/Mouse GSK-3 alpha / beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CDK1 bridges NF-kappaB and beta-catenin signaling in response to H.ï¿½pylori infection in gastric tumorigenesis
Authors: S Zhu, M Al-Mathkou, L Cao, S Khalafi, Z Chen, J Poveda, D Peng, H Lu, M Soutto, T Hu, OG McDonald, A Zaika, W El-Rifai
Cell Reports, 2023-01-21;42(1):112005.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer
Authors: Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden
Breast Cancer Research