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Human/Mouse EED Antibody

Catalog #: AF5827
5 Citations Datasheet
Catalog # Availability Size / Price Qty
AF5827
AF5827-SP
Detection of Human EED by Western Blot.
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Citations (5)
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Human/Mouse EED Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse EED in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EED
Ser2-Cys150
Accession # O75530
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EED antibody by Western Blot. View Larger

Detection of Human EED by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT-29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5827) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EED at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EED

EED (Embryonic ectoderm development; also WAIT-1) is a 51 kDa member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441 amino acids in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa 91‑441). There are two alternate start sites, one at Met15, and another that is 94 aa upstream of the standard site, begins with a Val and generates a 69 kDa isoform. A third isoform variant shows an insertion of 25 aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.

Long Name
Embryonic Ectoderm Development
Entrez Gene IDs
8726 (Human); 13626 (Mouse); 293104 (Rat)
Alternate Names
EED; embryonic ectoderm development; HEED; polycomb protein EED; WAIT1 ; WAIT1; WAIT-1; WD protein associating with integrin cytoplasmic tails 1

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Citations for Human/Mouse EED Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach
    Authors: VanDyke, D;Xu, L;Sargunas, PR;Gilbreth, RN;Baca, M;Gao, C;Hunt, J;Spangler, JB;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. An EZH2-NF-kappa B regulatory axis drives expression of pro-oncogenic gene signatures in triple negative breast cancer
    Authors: Gabrielle J. Dardis, Jun Wang, Jeremy M. Simon, Gang Greg Wang, Albert S. Baldwin
    iScience
  3. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader
    Authors: Frances Potjewyd, Anne-Marie W. Turner, Joshua Beri, Justin M. Rectenwald, Jacqueline L. Norris-Drouin, Stephanie H. Cholensky et al.
    Cell Chemical Biology
    Applications: Simple Western
  4. Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids
    Authors: EM Michalak, MJG Milevskiy, RM Joyce, JF Dekkers, PR Jamieson, B Pal, CA Dawson, Y Hu, SH Orkin, WS Alexander, GJ Lindeman, GK Smyth, JE Visvader
    PLoS Biol., 2018-08-06;16(8):e2004986.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Differential expression profiles of conserved Snail transcription factors in the mouse testis
    Authors: DJ Micati, GR Hime, EA McLaughlin, HE Abud, KL Loveland
    Andrology, 2018-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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