Human/Mouse Cyclin D1/D2 Antibody Summary
Met1-Ile295
Accession # P24385
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse Cyclin D1/D2 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Cyclin D1/D2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D1/D2
The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G0/G1 to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.
Product Datasheets
Citations for Human/Mouse Cyclin D1/D2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer
Authors: Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al.
International Journal of Oncology
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Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1
Authors: Wolf, B;Muralidharan, P;Lee, MY;Hua, W;Green, E;Wang, H;Strange, C;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Western Blot, ICC -
Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway
Authors: Wolf, B;Muralidharan, P;Lee, M;Hua, W;Green, E;Wang, H;Strange, C;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells, Protein
Applications: Western Blot, Immunocytochemistry/ Immunofluorescence -
Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p
Authors: XF Liu, K Wang, HC Du
Eur Rev Med Pharmacol Sci, 2020-07-01;24(14):7598-7611.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion
Authors: YJ Liu, W Li, F Chang, JN Liu, JX Lin, DX Chen
Oncol. Rep., 2017-12-11;39(2):491-500.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation.
Authors: Muruganandan S, Govindarajan R, McMullen N, Sinal C
Stem Cells, 2016-11-11;35(3):711-724.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
Authors: Q Chen, S Qin, Y Liu, M Hong, CN Qian, ET Keller, J Zhang, Y Lu
Oncotarget, 2016-10-18;7(42):68140-68150.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/beta-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci.
Authors: MacLeod R
Lab Invest, 2013-04-01;93(5):520-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
J. Biol. Chem., 2011-05-14;286(27):23982-95.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A Novel Nanofiber Hydrogel Loaded with Platelet-Rich Plasma Promotes Wound Healing Through Enhancing the Survival of Fibroblasts
Authors: Peng Zhang, Long Zhou, Lei Wang, Qirong Dong
Medical Science Monitor
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