Home / CXCL12/SDF-1 / Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

Catalog #: IC350F
3 Citations Datasheet
Catalog # Availability Size / Price Qty
IC350F
Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 79018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CXCL12/SDF-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Osteoblast Sorting and Intracellular Staining of CXCL12
    Authors: Weihuan Wang, Gurnoor Majihail, Cui Lui, Lan Zhou
    BIO-PROTOCOL
  2. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
    Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
    Proc Natl Acad Sci U S A, 2020-10-30;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Long-term B cell depletion in murine lupus eliminates autoantibody-secreting cells and is associated with alterations in the kidney plasma cell niche.
    Authors: Wang, Wensheng, Rangel-Moreno, Javier, Owen, Teresa, Barnard, Jennifer, Nevarez, Sarah, Ichikawa, H Travis, Anolik, Jennifer
    J Immunol, 2014-02-26;192(7):3011-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody and earn rewards!

Have you used Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review