Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody Summary
CRGTELDCGIETD
Accession # U26943
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC835G, filled histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Caspase-3
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
Product Datasheets
Citations for Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Apoptosis Induction in HepG2 and HCT116 Cells by a Novel Quercetin-Zinc (II) Complex: Enhanced Absorption of Quercetin and Zinc (II)
Authors: Nakamura, M;Urakawa, D;He, Z;Akagi, I;Hou, DX;Sakao, K;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: ICC -
EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury
Authors: H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva
Mol. Ther., 2018-06-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
Haematologica, 2017-12-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth
Authors: Chunyan Zhang, Chanyu Yue, Andreas Herrmann, Jieun Song, Colt Egelston, Tianyi Wang et al.
Cell Metabolism
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody
Average Rating: 3.5 (Based on 2 Reviews)
Have you used Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
