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Human/Mouse CD27/TNFRSF7 Antibody

Catalog #: AF574
1 Citation Datasheet
Catalog # Availability Size / Price Qty
AF574
AF574-SP
Detection of Human and Mouse CD27/TNFRSF7 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CD27/TNFRSF7 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CD27/TNFRSF7 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
Thr21-Arg182
Accession # P41272
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse CD27/TNFRSF7 antibody by Western Blot. View Larger

Detection of Human and Mouse CD27/TNFRSF7 by Western Blot. Western blot shows lysates of human lymph node tissue and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD27/TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD27/TNFRSF7 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD27/TNFRSF7 in Mouse Splenocytes. CD27/TNFRSF7 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF574) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27 is a lymphocyte-specific member of the tumor necrosis factor receptor superfamily (TNFRSF) and is designated TNFRSF7 (1, 2). Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region (3). Mouse and human CD27 share approximately 65% amino acid identity. CD27 exists as homodimers on the cell surface via an extracellular disulfide bond in the membrane-proximal region. A soluble form of CD27 is also produced during the immune response and is found in various body fluids (4). CD27 is expressed on subsets of T and B cells. The expression of CD27 is upregulated upon T-cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells (5). CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (1, 2). Ligation of CD27 on B cells has been shown to inhibit terminal differentiation of activated mouse B cells into plasma cells and enhances commitment to memory B cell responses (5).

References
  1. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  2. Croft, M. (2003) Cytokine and Growth Factor Reviews 14:265.
  3. Gravestein, L.A. et al. (1993) Eur. J. Immunol. 23:943.
  4. Lens, S.M. et al. (1998) Semin. Immunol. 10:491.
  5. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
Entrez Gene IDs
939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
Alternate Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Citation for Human/Mouse CD27/TNFRSF7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dimerization Interface of Osteoprotegerin Revealed by Hydrogen-deuterium Exchange Mass Spectrometry
    Authors: Y Xiao, M Li, R Larocque, F Zhang, A Malhotra, J Chen, RJ Linhardt, L Konermann, D Xu
    J. Biol. Chem., 2018-09-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)

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