Human/Mouse Cathepsin L Antibody Summary
Glu113-Val333
Accession # P07711
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human and Mouse Cathepsin L by Western Blot. Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515.
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Cathepsin L in Human Kidney. Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cathepsin L in Mouse Kidney. Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin L
Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3).
- Goretzki, L. et al. (1992) FEBS Lett. 297:112.
- Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
- Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
Product Datasheets
Citations for Human/Mouse Cathepsin L Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury
Authors: Y Li, JW Jones, H M C Choi, C Sarkar, MA Kane, EY Koh, MM Lipinski, J Wu
Cell Death Dis, 2019-07-11;10(7):531.
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PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma
Authors: C Sarkar, JW Jones, N Hegdekar, JA Thayer, A Kumar, AI Faden, MA Kane, MM Lipinski
Autophagy, 2019-06-25;0(0):1-20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
CCT complex restricts neuropathogenic protein aggregation via autophagy
Nat Commun, 2016-12-08;7(0):13821.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy
Sci Adv, 2016-09-02;2(9):e1601167.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
Cell Death Differ, 2014-06-06;21(9):1442-50.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
A 3-D in vitro co-culture model of mammary gland involution
Authors: Jonathan J. Campbell, Laur-Alexandru Botos, Timothy J. Sargeant, Natalia Davidenko, Ruth E. Cameron, Christine J. Watson
Integr Biol (Camb)
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Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution.
Authors: O'Brien J, Martinson H, Durand-Rougely C
Development, 2011-11-30;139(2):269-75.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Stat3 controls lysosomal-mediated cell death in vivo.
Authors: Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ
Nat. Cell Biol., 2011-02-20;13(3):303-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases.
Authors: Lopez-Castejon G, Theaker J, Pelegrin P
J. Immunol., 2010-07-16;185(4):2611-9.
Species: Human, Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
FASEB J., 2010-05-21;24(10):3744-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer.
Authors: Parker BS, Ciocca DR, Bidwell BN, Gago FE, Fanelli MA, George J, Slavin JL, Moller A, Steel R, Pouliot N, Eckhardt BL, Henderson MA, Anderson RL
J. Pathol., 2008-02-01;214(3):337-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification.
Authors: Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL
J. Biol. Chem., 2006-03-24;281(23):15893-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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