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Human/Mouse Bcl-x Antibody

Catalog #: AF800
11 Citations Datasheet
Catalog # Availability Size / Price Qty
AF800
AF800-SP
Detection of Human and Mouse Bcl‑x by Western Blot.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bcl-x Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl‑x in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below
Simple Western
3 µg/mL
See below
Immunoprecipitation
5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Bcl-x antibody by Western Blot. View Larger

Detection of Human and Mouse Bcl‑x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunoprecipitation Immunoprecipitation of Human Bcl-x. View Larger

Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.

Simple Western Detection of Human and Mouse Bcl‑x antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse Bcl‑x by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-x

The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.

Long Name
B Cell Lymphoma/Leukemia x
Entrez Gene IDs
598 (Human); 12048 (Mouse); 24888 (Rat)
Alternate Names
Apoptosis regulator Bcl-X;BCL2L;BCL2L1;Bcl-2-like protein 1;BCLX;Bcl-X;BCL-XL/S;PPP1R52; BCL2L1; Bclx; Bcl-x

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Citations for Human/Mouse Bcl-x Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Mimicking Cdk2 phosphorylation of Bcl-xL at Ser73 results in caspase activation and Bcl-xL cleavage
    Authors: N S Seng, J Megyesi, A Tarcsafalvi, P M Price
    Cell Death Discovery
  2. Cdk2 phosphorylation of Bcl-xL after stress converts it to a pro-apoptotic protein mimicking Bax/Bak
    Authors: J Megyesi, A Tarcsafalvi, NSHL Seng, R Hodeify, P M Price
    Cell Death Discovery
  3. LOXL1 confers antiapoptosis and promotes gliomagenesis through stabilizing BAG2
    Authors: Yu H, Ding J, Zhu H et al.
    Cell Death Differ.
  4. The Apoptotic Effect of Caffeic or Chlorogenic Acid on the C32 Cells That Have Simultaneously Been Exposed to a Static Magnetic Field
    Authors: M Kimsa-Dude, A Synowiec-W, A Krawczyk, A Kosowska, M Kimsa-Furd, T Francuz
    International Journal of Molecular Sciences, 2022-03-31;23(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Carbon ion triggered immunogenic necroptosis of nasopharyngeal carcinoma cells involving necroptotic inhibitor BCL-x
    Authors: C Bao, Y Sun, B Dwarakanat, Y Dong, Y Huang, X Wu, C Guha, L Kong, JJ Lu
    Journal of Cancer, 2021-01-01;12(5):1520-1530.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.
    Authors: Schmitt E, Beauchemin M, Bertrand R
    Oncogene, 2007-03-19;26(40):5851-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  7. Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
    Authors: Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
    Clin. Cancer Res., 2004-11-01;10(21):7427-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  8. Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
    Authors: Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
    Clin. Cancer Res., 2004-03-15;10(6):2100-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
    Authors: Raman VS, Akondy RS, Rath S, Bal V, George A
    J. Immunol., 2003-12-01;171(11):5876-81.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2003-10-28;279(4):2937-44.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
    Authors: Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
    J. Exp. Med., 2002-07-01;196(1):87-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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