Human/Mouse Bcl-w Antibody Summary
Gly32-Asp51
Accession # Q92843
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Bcl‑w by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-w
Bcl-w, also called Bcl-2 like protein 2, is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
- Ross, J.A., et al. (1998) Nat. Genet. 18:251.
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Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
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Yan, W. et al. (2000) Mol. Endocrin. 14:682.
Product Datasheets
Citations for Human/Mouse Bcl-w Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
Authors: D Aird, T Teng, CL Huang, E Pazolli, D Banka, K Cheung-Ong, C Eifert, C Furman, ZJ Wu, M Seiler, S Buonamici, P Fekkes, C Karr, J Palacino, E Park, PG Smith, L Yu, Y Mizui, M Warmuth, A Chicas, L Corson, P Zhu
Nat Commun, 2019-01-11;10(1):137.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Authors: Jayanthi S, åÏctÐs, Deng X, Bordelon M, McCoy MT, Cadet JL
2001-08-01;15(10):1745-52.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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