Human/Mouse Bad aa 1‑21 Antibody Summary
MFQIPEFEPSEQEDSSSAERGC
Accession # Q92934
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse Bad by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Bad in Jurkat Human Cell Line. Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bad
Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.
Product Datasheets
Citations for Human/Mouse Bad aa 1‑21 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
PLoS ONE, 2014-06-13;9(6):e99586.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes.
Authors: Xi H, Kersh GJ
J. Immunol., 2004-07-01;173(1):340-8.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Translocation of full-length Bid to mitochondria during anoikis.
Authors: Valentijn AJ, Gilmore AP
J. Biol. Chem., 2004-05-17;279(31):32848-57.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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