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Human MMP-8 Antibody

Catalog #: MAB9081
3 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9081
MAB9081-SP
MMP‑8 in Human Breast Cancer Tissue.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects both pro and active forms of human MMP-8 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -3, -7, -9, -10, -12, or -13 is observed.
Source
Monoclonal Mouse IgG2A Clone # 100608
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑8
Phe21-Gly467
Accession # AAZ38714
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB908).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total MMP-8 DuoSet ELISA Kit (Catalog # DY908) for convenient development of a sandwich ELISA or the Human Total MMP-8 Quantikine ELISA Kit (Catalog # DMP800) for a complete optimized ELISA.

 
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MMP‑8 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑8 in Human Breast Cancer Tissue. MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human MMP-8 Monoclonal Antibody (Catalog # MAB9081) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in terminal ductules (round) and intralobular duct (elongated) composing glandular lobules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ELISA View Larger

Human MMP-8 ELISA Standard Curve. Recombinant Human MMP‑8 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP‑8 Monoclonal Antibody (MAB908) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB9081) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Intracellular Staining by Flow Cytometry View Larger

Detection of MMP‑8 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB9081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.

Long Name
Matrix Metalloproteinase 8
Entrez Gene IDs
4317 (Human); 17394 (Mouse); 63849 (Rat)
Alternate Names
CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

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Citations for Human MMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Systematic Evaluation of the Cellular Innate Immune Response During the Process of Human Atherosclerosis.
    Authors: van Dijk R A, Rijs K et al.
    J Am Heart Assoc
  2. Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease
    Authors: Pim J Koelink, Saskia A Overbeek, Saskia Braber, Mary E Morgan, Paul A J Henricks, Mojtaba Abdul Abdul Roda et al.
    Gut
  3. Matrix metalloproteinase-8 is expressed in human chorion during labor.
    Authors: Arechavaleta-Velasco F, Marciano D, Diaz-Cueto L, Parry S
    Am. J. Obstet. Gynecol., 2004-03-01;190(3):843-50.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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