Human MMP-7 Antibody Summary
Leu18-Lys267
Accession # P09237
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MMP‑7 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MMP‑7 in Human Pancreas. MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human MMP‑7 by Simple WesternTM. Simple Western lane view shows lysates of normal stem cells (negative control) and Crohn's stem cells, loaded at 0.2 mg/mL. A specific band was detected for MMP-7 at approximately 10 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Neutralization of MMP‑7 Activity by Human MMP‑7 Antibody. The cleavage of Mca-PLGL-Dpa-AR-NH2(10 µM, Catalog # ES001) by Recombinant Human MMP-7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND50 is typically 2 µg/mL.
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Detection of MMP‑7 in Human Pancreas. Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for MMP7 mRNA (ACD RNAScope Probe, catalog #488408; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human MMP7 polyclonal antibody (R&D Systems catalog # AF907) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-7
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal
alpha ‑defensin activation in innate host defense, releases tumor necrosis factor‑ alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
Product Datasheets
Citations for Human MMP-7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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MMP7 interacts with ARF in nucleus to potentiate tumor microenvironments for prostate cancer progression in vivo
Authors: Yingqiu Xie, Wenfu Lu, Shenji Liu, Qing Yang, J. Shawn Goodwin, Sandeep Anantha Sathyanarayana et al.
Oncotarget
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Hypomethylation of the MMP7 promoter and increased expression of MMP7 distinguishes the basal-like breast cancer subtype from other triple-negative tumors
Authors: Steven T. Sizemore, Gina M. Sizemore, Christine N. Booth, Cheryl L. Thompson, Paula Silverman, Gurkan Bebek et al.
Breast Cancer Research and Treatment
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Kidney Damage in Long COVID: Studies in Experimental Mice
Authors: Ramamoorthy, R;Hussain, H;Ravelo, N;Sriramajayam, K;Di Gregorio, DM;Paulrasu, K;Chen, P;Young, K;Masciarella, AD;Jayakumar, AR;Paidas, MJ;
Biology
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: Western Blot, IHC -
Matrix metalloproteinase-7 could be a predictor for acute inflammation in psoriatic patients
Authors: A Abbes, Y Zayani, W Zidi, MB Hammami, A Mebazaa, D El Euch, A Ben Ammar, H Sanhaji, MV El May, M Mokni, M Feki, M Allal-Elas
Cytokine, 2020-07-11;134(0):155195.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Farnesoid X receptor represses matrix metalloproteinase 7 expression, revealing this regulatory axis as a promising therapeutic target in colon cancer
Authors: Z Peng, J Chen, CB Drachenber, JP Raufman, G Xie
J. Biol. Chem., 2019-04-09;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade.
Authors: Zhuo, Jingli, Tan, Ee Hong, Yan, Benedict, Tochhawng, Lalchhan, Jayapal, Manikand, Koh, Shiuan, Tay, Hwee Kee, Maciver, Sutherla, Hooi, Shing Ch, Salto-Tellez, Manuel, Kumar, Alan Pre, Goh, Yaw Chon, Lim, Yaw Chyn, Yap, Celestia
PLoS ONE, 2012-08-21;7(8):e43594.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Determination of matrilysin activity in gastrointestinal neoplasia.
Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF
Eur. J. Clin. Invest., 2007-07-01;37(7):598-9.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Heparanase-Regulated Syndecan-1 Shedding Facilitates Herpes Simplex Virus 1 Egress
Authors: Satvik Hadigal, Raghuram Koganti, Tejabhiram Yadavalli, Alex Agelidis, Rahul Suryawanshi, Deepak Shukla
Journal of Virology
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