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Human MMP-7 Antibody

Catalog #: MAB9071
18 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9071-SP
MAB9071-500
MAB9071-100
Detection of Human MMP‑7 by Western Blot.
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Product Details
Citations (18)
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Human MMP-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects the pro and active forms of human MMP-7 in Western blots. In dot blots, approximately 20% cross-reactivity with recombinant human MMP-8 is observed and no cross-reactivity with recombinant human MMP-1, -2, -3, -9, -10, -12, or -13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 111433
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑7
Leu18-Lys267 (Ala230del)
Accession # NP_002414.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑7 (Catalog # 907-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MMP-7 antibody by Western Blot. View Larger

Detection of Human MMP‑7 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human MMP-7 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human MMP‑7 by Simple WesternTM. Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected for MMP‑7 at approximately 49 and 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human MMP-7 by Western Blot View Larger

Detection of Human MMP-7 by Western Blot Zymography analysis for active MMP2 was performed with 4 μg of total protein of the conditioned medium of U87 (a) and primary IC cells (b) after 48 h exposure to JS-K (1–3 μM) on gelatin-containing PAGE. Western blot analysis for MMP2, MMP7, MMP9 and TIMP3 was performed for conditioned medium of U87 (a) and IC (b) cells with 4 μg of total protein as well as of 20 μg of total cell lysate of U87 (c) and IC cells (d) on SDS-PAGE. Conditioned media of knockdown and overexpression of ATF3 were examined for active MMP2 as well as the plasmid controls and uninfected U87 (e). 4 μg of protein of the supernatant (e) and 20 μg of cell lysates (f) were screened for MMP2, MMP7, MMP9 and TIMP3 by SDS-PAGE. Coomassie staining of the gels was used as a loading control for zymogram and for western blot of the supernatant, GAPDH was used as a loading control for cell lysates. Figures are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28250971), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Long Name
Matrix Metalloproteinase 7
Entrez Gene IDs
4316 (Human); 17393 (Mouse)
Alternate Names
EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase

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Citations for Human MMP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker
    Authors: M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström
    Cancers, 2022-07-13;14(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Fecal host biomarkers predicting severity of Clostridioides difficile infection
    Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
    JCI Insight, 2021-01-11;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Upregulation of long non?coding RNA CCEPR is associated with poor prognosis and contributes to the progression of ovarian cancer through regulating the Wnt/beta?catenin signaling pathway
    Authors: Z Chen, Y Zhu, X Fan, Y Liu, Q Feng
    Mol Med Rep, 2020-02-06;21(4):1950-1958.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Silencing microRNA-330-5p increases MMP1 expression and promotes an invasive phenotype in oesophageal adenocarcinoma
    Authors: Becky A. S. Bibby, Cecelia S. Miranda, John V. Reynolds, Christopher J. Cawthorne, Stephen G. Maher
    BMC Cancer
  5. miR-126-3p down-regulation contributes to dabrafenib acquired resistance in melanoma by up-regulating ADAM9 and VEGF-A
    Authors: S Caporali, A Amaro, L Levati, E Alvino, PM Lacal, S Mastroeni, F Ruffini, L Bonmassar, GC Antonini C, N Felli, A Carè, U Pfeffer, S D'Atri
    J. Exp. Clin. Cancer Res., 2019-06-21;38(1):272.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Coexpression of p-IGF-1R and MMP-7 Modulates Panitumumab and Cetuximab Efficacy in RAS Wild-Type Metastatic Colorectal Cancer Patients
    Authors: V Alonso, P Escudero, C Fernández-, A Salud, M Méndez, J Gallego, JR Rodriguez, M Martín-Ric, J Fernández-, H Manzano, JC Méndez, M Zanui, E Falcó, M Gil-Raga, F Rojo, M Cuatrecasa, J Feliu, X García-Alb, J Maurel
    Neoplasia, 2018-05-26;20(7):678-686.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Decreased Invasion of Urothelial Carcinoma of the Bladder by Inhibition of Matrix-Metalloproteinase 7
    Authors: C Bolenz, D Knauf, A John, P Erben, A Steidler, SW Schneider, C Günes, C Gorzelanny
    Bladder Cancer, 2018-01-20;4(1):67-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NF?B and STAT3 inhibition in glioblastoma
    Authors: J Guenzle, LJ Wolf, NW Garrelfs, JM Goeldner, N Osterberg, CR Schindler, JE Saavedra, A Weyerbrock
    Cell Death Discov, 2017-02-27;3(0):17006.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. AP2 alpha controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression
    Authors: N Felli, M C Errico, F Pedini, M Petrini, R Puglisi, M Bellenghi et al.
    Oncogene
  10. Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces.
    Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B
    J. Cell. Sci., 2011-01-11;124(0):369-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
    Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S
    BJU Int., 2010-09-03;107(7):1069-73.
    Species: Human
    Sample Types: Urine
    Applications: Immunoprecipitation
  12. Pathway-Focused Arrays Reveal Increased Matrix Metalloproteinase-7 (Matrilysin) Transcription in Trachomatous Trichiasis
    Authors: Martin J. Holland, David Jeffries, Michael Pattison, Gerit Korr, Alevtina Gall, Hassan Joof et al.
    Investigative Opthalmology & Visual Science
  13. Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
    Authors: Szarvas T, Becker M, vom Dorp F, Gethmann C, Totsch M, Bankfalvi A, Schmid KW, Romics I, Rubben H, Ergun S
    Cancer Sci., 2010-01-20;101(5):1300-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
    Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
    J. Mol. Cell. Cardiol., 2009-05-03;47(2):296-303.
    Species: Rabbit
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  15. Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma.
    Authors: Goswami S, Angkasekwinai P, Shan M, Greenlee KJ, Barranco WT, Polikepahad S, Seryshev A, Song LZ, Redding D, Singh B, Sur S, Woodruff P, Dong C, Corry DB, Kheradmand F
    Nat. Immunol., 2009-03-29;10(5):496-503.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery.
    Authors: Yu KH, Barry CG, Austin D
    J. Proteome Res., 2009-03-01;8(3):1565-76.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  17. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  18. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007-05-01;178(9):5957-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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