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Human MMP-3 Antibody

Catalog #: MAB905
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB905
MAB905-SP
MMP‑3 in Human Ovarian Cancer Tissue.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects the pro and active forms of human MMP-3 in Western blots. In Western blots, no cross-reactivity with recombinant human
(rh) MMP‑1, rhMMP-2 or rhMMP-9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 10D6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑3 Western Blot Standard (Catalog # WBC015)
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑3 (Catalog # 513-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MMP‑3 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑3 in Human Ovarian Cancer Tissue. MMP-3 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB905) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-3

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Long Name
Matrix Metalloproteinase 3
Entrez Gene IDs
4314 (Human); 17392 (Mouse)
Alternate Names
CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1

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Citations for Human MMP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Higher Content but Not Activity of Stromelysin-2 (MMP-10) in Comparison to Stromelysin-1 (MMP-3) in Human Renal Carcinoma
    Authors: J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, L Romanowicz
    International Journal of Environmental Research and Public Health, 2022-10-02;19(19):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma
    Authors: J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, K Sobolewski, L Romanowicz
    Journal of Clinical Medicine, 2021-08-19;10(16):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  4. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007-01-01;48(6):300-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Dot Blot
  5. Expression and activity of matrix metalloproteases in human malignant mesothelioma cell lines.
    Authors: Liu Z, Ivanoff A, Klominek J
    Int J Cancer, 2001-03-01;91(5):638-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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