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Human MMP-2 Antibody

Catalog #: MAB902
13 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB902
MAB902-SP
MMP‑2 in Human Ovarian Cancer Tissue.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 36006
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025) under non-reducing conditions only
Immunohistochemistry
50-100 µg/mL
See below
Dual RNAscope ISH-IHC
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human stomach

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑2 in Human Ovarian Cancer Tissue. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑2 in Human Ovarian Cancer Tissue. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

In-situ Hybridization View Larger

Detection of MMP‑2 in Human Stomach. Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for MMP2 mRNA (ACD RNAScope Probe, catalog #311751; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human MMP2 monoclonal antibody (R&D Systems catalog # MAB902) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

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Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010-03-01;126(5):1095-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010-01-14;82(5):968-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  3. High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
    Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
    Infect. Immun., 2009-12-14;78(3):1012-21.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
    Authors: Schneider P, Costa O, Legrand E
    Leuk. Res., 2009-09-12;34(1):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
    Authors: Ooi YH, Oh DJ, Rhee DJ
    Invest. Ophthalmol. Vis. Sci., 2009-05-14;50(11):5259-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Urinary biomarkers predict brain tumor presence and response to therapy.
    Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
    Clin. Cancer Res., 2008-04-15;14(8):2378-86.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
    Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
    J. Pathol., 2008-03-01;214(4):464-71.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
    Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
    Surgery, 2006-09-01;140(3):454-63.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Oxidized low-density lipoproteins stimulate extracellular matrix metalloproteinase Inducer (EMMPRIN) release by coronary smooth muscle cells.
    Authors: Haug C, Lenz C, Diaz F, Bachem MG
    Arterioscler. Thromb. Vasc. Biol., 2004-08-19;24(10):1823-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. Expression of matrix metalloproteinases 2, 7 and 9 in patients with colorectal cancer
    Authors: Elena Kostova, Maja Slaninka-Miceska, Nikola Labacevski, Krume Jakovski, Jasmina Trojachanec, Emilija Atanasovska et al.
    Vojnosanitetski pregled
  11. Blockade of monocyte-endothelial trafficking by transduced Tat-superoxide dismutase protein
    Authors: SIN-HYE PARK, MIN JAE SHIN, DAE WON KIM, JINSEU PARK, SOO YOUNG CHOI, YOUNG-HEE KANG
    International Journal of Molecular Medicine
  12. Orexin-A Exerts Equivocal Role in Atherosclerosis Process Depending on the Duration of Exposure: In Vitro Study
    Authors: Narjes Nasiri Ansari, Flora Spentza, Georgios K. Dimitriadis, Aphrodite Daskalopoulou, Angeliki Karapanagioti, Gerasimos Siasos et al.
    Nutrients
  13. MMP-2 Isoforms in Aortic Tissue and Serum of Patients with Ascending Aortic Aneurysms and Aortic Root Aneurysms
    PLoS ONE, 2016-11-01;11(11):e0164308.

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