Human MMP-2 Antibody

Catalog #: MAB9022
3 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
MAB9022
MAB9022-SP

All MMP-2 Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects the pro and active forms of human MMP-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 101724

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

MMP-2, also called gelatinase A, is a matrix metalloproteinase that can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. MMP-2 is produced by neutrophils, macrophages and monocytes.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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JNK and p38 Inhibitors Prevent Transforming Growth Factor-&beta1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts
Authors: TY Hou, SB Wu, HC Kau, CC Tsai
International Journal of Molecular Sciences, 2021-03-14;22(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2008-12-16;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006-05-08;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot