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Human MMP-2 Antibody

Catalog #: MAB9021
3 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9021
MAB9021-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects pro and active forms of human MMP-2. In direct ELISAs, 100% cross-reactivity with recombinant mouse MMP-2 is observed. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 101721
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP‑1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP‑2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP‑2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

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Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The Significance of Matrix Metalloproteinase 9 (MMP-9) and Metalloproteinase 2 (MMP-2) in Urinary Bladder Cancer
    Authors: J Kudelski, A Tokarzewic, M Gudowska-S, B Mroczko, P Ch?osta, M Bruczko-Go, P Mitura, G M?ynarczyk
    Biomedicines, 2023-03-20;11(3):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells
    Authors: GB Kim, JM Lee, DL Nguyen, J Lee, YP Kim
    International Journal of Molecular Sciences, 2022-01-30;23(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007-05-01;178(9):5957-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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