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Human MMP-10 Antibody

Catalog #: MAB9101
6 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB9101
MAB9101-SP
Detection of Human MMP-10 by Immunohistochemistry
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Product Details
Citations (6)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects the pro and active forms of human MMP-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 117239
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-10
Tyr18-Cys476
Accession # P09238
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑10 Western Blot Standard (Catalog # WBC026)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human intestine
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human MMP‑10 (1 µg/mL, Catalog # 910‑MP) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 1 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of Human MMP-10 by Immunohistochemistry View Larger

Detection of Human MMP-10 by Immunohistochemistry Co-localisation of MMPs with markers of classical activation in human atherosclerotic plaques.(A) Serial sections were stained by peroxidase with anti-CD68 (A. CD68) and by dual immuno-fluorescence with anti-MMP-1 or anti-MMP-10 (red) together with anti-COX-2 or p65RelA (green) as shown, and the images were superimposed digitally. Nuclear localised p65RelA (NF-kB*) was detected because of the shift in nuclear counterstain colour from dark blue (DAPI alone) to sky blue (blue plus green) in the superimposed image. (B) Areas rich in macrophages identified from the peroxidase stain were identified in the serial section. Cells in the whole field were counted and a percentage of each staining pattern calculated. Values are means ± SEM, n = 6, * p<0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22880008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-10

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 10
Entrez Gene IDs
4319 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.22; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP10; MMP-10; SL-2; STMY2stromelysin-2; Stromelysin 2; transin 2; transin-2

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Citations for Human MMP-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. MMP-10 from M1 macrophages promotes pulmonary vascular remodeling and pulmonary arterial hypertension
    Authors: PL Chi, CC Cheng, CC Hung, MT Wang, HY Liu, MW Ke, MC Shen, KC Lin, SH Kuo, PP Hsieh, SR Wann, WC Huang
    International journal of biological sciences, 2022-01-01;18(1):331-348.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Combined sustained release of BMP2 and MMP10 accelerates bone formation and mineralization of calvaria critical size defect in mice
    Authors: R Reyes, JA Rodríguez, J Orbe, MR Arnau, C Évora, A Delgado
    Drug Deliv, 2018-11-01;25(1):750-756.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
    Authors: Ye Y, Pringle LM, Lau AW
    Oncogene, 2010-04-26;29(25):3619-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006-10-09;175(1):179-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inside the Thrombus: Association of Hemostatic Parameters With Outcomes in Large Vessel Stroke Patients
    Authors: Juan Marta-Enguita, Manuel Navarro-Oviedo, Roberto Muñoz, Jorge Olier-Arenas, Guillermo Zalba, Ramon Lecumberri et al.
    Frontiers in Neurology
  6. Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.
    Authors: Saghizadeh M, Kramerov AA, Yaghoobzadeh Y et al.
    Brain Res Bull

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Human MMP-10 Antibody
By Anonymous on 03/13/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human
Immunocytochemistry/Immunofluorescence Human MMP-10 Antibody MAB9101