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Human MIF Quantikine ELISA Kit

Catalog #: DMF00B
32 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
SMF00B
DMF00B
PDMF00B
Control Products Available
QC122: Quantikine Immunoassay Control Set 985 for Human MIF
Human MIF ELISA Standard Curve
2 Images
Product Details
Citations (32)
FAQs
Supplemental Products
Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human MIF Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
0.068 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human MIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.16 3.56 7.27 1.03 3.2 6.46
Standard Deviation 0.07 0.18 0.33 0.1 0.27 0.61
CV% 6 5.1 4.5 9.7 8.4 9.4

Recovery

The recovery of MIF spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 86-104
Platelet-poor EDTA Plasma (n=5) 95 80-108
Platelet-poor Heparin Plasma (n=5) 96 86-108
Serum (n=4) 99 94-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human MIF ELISA Linearity

Scientific Data

N/A Human MIF ELISA Standard Curve View Larger

Human MIF ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIF

MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

Long Name:
Macrophage Migration Inhibitory Factor
Entrez Gene IDs:
4282 (Human); 17319 (Mouse)
Alternate Names:
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human MIF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. The level of macrophage migration inhibitory factor is negatively correlated with the efficacy of PD-1 blockade immunotherapy combined with chemotherapy as a neoadjuvant therapy for esophageal squamous cell carcinoma
    Authors: Wu, L;Gao, Y;Xie, S;Ye, W;Uemura, Y;Zhang, R;Yu, Y;Li, J;Chen, M;Wu, Q;Cui, P;Liu, H;Mu, S;Li, Y;Wang, L;Liu, C;Li, J;Zhang, L;Jiao, S;Zhang, G;Liu, T;
    Translational oncology
    Species: Human
    Sample Types: Serum
  2. A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol
    Authors: MS Simon, BB Barton, C Glocker, R Musil
    PLoS ONE, 2022-07-21;17(7):e0271793.
    Species: Human
    Sample Types: Plasma
  3. MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment
    Authors: L Garcia-Ger, M García, A Garrido-Ga, S Gómez-Gonz, M Torrebadel, E Prada, G Pascual-Pa, O Muñoz, S Perez-Jaum, I Lemos, N Salvador, M Vila-Ubach, A Doncel-Req, M Suñol, AM Carcaboso, J Mora, C Lavarino
    BMC Cancer, 2022-06-17;22(1):669.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
    Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
    Cell Death & Disease, 2022-06-06;13(6):527.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Macrophage migration inhibitory factor in Nodding syndrome
    Authors: G Benedek, M Abed El La, K Miller, M Rivkin, A Ahmed Rama, L P Riek, R Lako, S Edvardson, S Arbel-Alon, E Galun, M Levite
    PloS Neglected Tropical Diseases, 2021-10-18;15(10):e0009821.
    Species: Human
    Sample Types: Plasma
  6. Preoperative Serum Macrophage Migration Inhibitory Factor Level Correlates with Surgical Difficulty and Outcome in Patients with Autoimmune Thyroiditis
    Authors: KH Chong, MH Wu, CW Chen, TH Hsieh, CW Lai
    Journal of Clinical Medicine, 2021-09-07;10(18):.
    Species: Human
    Sample Types: Serum
  7. Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
    Authors: HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um
    Cells, 2020-05-28;9(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. MIF gene rs755622 polymorphism positively associated with acute coronary syndrome in Chinese Han population: case-control study
    Authors: GL Du, JY Luo, D Wang, YH Li, BB Fang, XM Li, XM Gao, YN Yang
    Sci Rep, 2020-01-10;10(1):140.
    Species: Human
    Sample Types: Plasma
  9. Increased serum levels of macrophage migration inhibitory factor in autism spectrum disorders
    Authors: J Ning, L Xu, CQ Shen, YY Zhang, Q Zhao
    Neurotoxicology, 2018-11-29;71(0):1-5.
    Species: Human
    Sample Types: Serum
  10. CSF macrophage migration inhibitory factor levels did not predict steroid treatment response after optic neuritis in patients with multiple sclerosis
    Authors: M Pawlitzki, CM Sweeney-Re, SG Meuth, D Reinhold, J Neumann
    PLoS ONE, 2018-11-26;13(11):e0207726.
    Species: Human
    Sample Types: CSF
  11. Macrophage migration inhibitory factor (MIF) is associated with degree of collateralization in patients with totally occluded coronary arteries
    Authors: L Di Serafin, J Bartunek, G Heyndrickx, K Dierickx, G Scognamigl, T Tesorio, B De Bruyne, B Trimarco, W Wijns, E Barbato
    Int. J. Cardiol., 2018-03-21;0(0):.
    Species: Human
    Sample Types: Plasma
  12. Increased serum levels and promoter polymorphisms of macrophage migration inhibitory factor in schizophrenia
    Authors: S Okazaki, A Hishimoto, I Otsuka, Y Watanabe, S Numata, S Boku, N Shimmyo, M Kinoshita, E Inoue, T Ohmori, T Someya, I Sora
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018-01-02;83(0):33-41.
    Species: Human
    Sample Types: Serum
  13. MIF and D-DT are potential disease severity modifiers in male MS subjects
    Authors: G Benedek, R Meza-Romer, K Jordan, Y Zhang, H Nguyen, G Kent, J Li, E Siu, J Frazer, M Piecychna, X Du, A Sreih, L Leng, J Wiedrick, SJ Caillier, H Offner, JR Oksenberg, V Yadav, D Bourdette, R Bucala, AA Vandenbark
    Proc. Natl. Acad. Sci. U.S.A., 2017-09-18;0(0):.
    Species: Human
    Sample Types: Plasma
  14. Comparison of macrophage migration inhibitory factor and neutrophil gelatinase-associated lipocalin-2 to predict acute kidney injury after liver transplantation: An observational pilot study
    Authors: J Baron-Stef, J Schiefer, EJ Miller, GA Berlakovic, DM Baron, P Faybik
    PLoS ONE, 2017-08-15;12(8):e0183162.
    Species: Human
    Sample Types: Serum
  15. Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane
    Authors: R Kumar, T de Mooij, TE Peterson, T Kaptzan, AJ Johnson, DJ Daniels, IF Parney
    PLoS ONE, 2017-06-30;12(6):e0179012.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
    Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
    J Neuroinflammation, 2017-06-24;14(1):126.
    Species: Human
    Sample Types: Serum
  17. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017-05-31;15(1):121.
    Species: Human
    Sample Types: Plasma
  18. Graft-derived macrophage migration inhibitory factor correlates with hepatocellular injury in patients undergoing liver transplantation
    Authors: J Baron-Stef, J Schiefer, EJ Miller, W Plöchl, CG Krenn, GA Berlakovic, DM Baron, P Faybik
    Clin Transplant, 2017-04-21;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  19. Temporal trend of autonomic nerve function and HSP27, MIF and PAI-1 in type 1 diabetes
    Authors: M Zimmerman, S Rolandsson, H Skärstrand, K Pourhamidi, A Gottsäter, P Wollmer, O Rolandsson, G Westergren, LB Dahlin
    J Clin Transl Endocrinol, 2017-03-20;8(0):15-21.
    Species: Human
    Sample Types: Serum
  20. MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
    Authors: Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
    Sci Rep, 2016-07-06;6(0):29160.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Effects of high intensity training and high volume training on endothelial microparticles and angiogenic growth factors.
    Authors: Wahl P, Jansen F, Achtzehn S, Schmitz T, Bloch W, Mester J, Werner N
    PLoS ONE, 2014-04-25;9(4):e96024.
    Species: Human
    Sample Types: Serum
  22. Significance of the vascular endothelial growth factor and the macrophage migration inhibitory factor in the progression of hepatocellular carcinoma.
    Authors: Wang D, Luo L, Chen W, Chen L, Zeng W, Li W, Huang X
    Oncol Rep, 2013-12-23;31(3):1199-204.
    Species: Human
    Sample Types: Serum
  23. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
    Authors: Kreutz M, Karrer S, Hoffmann P
    J. Invest. Dermatol., 2011-08-18;132(1):179-87.
    Species: Human
    Sample Types: Serum
  24. Serum macrophage migration-inhibitory factor as a diagnostic and prognostic biomarker for gastric cancer.
    Authors: Xia HH, Yang Y, Chu KM, Gu Q, Zhang YY, He H, Wong WM, Leung SY, Yuen ST, Yuen MF, Chan AO, Wong BC
    Cancer, 2009-12-01;115(23):5441-9.
    Species: Human
    Sample Types: Serum
  25. Identification of macrophage migration inhibitory factor and human neutrophil peptides 1-3 as potential biomarkers for gastric cancer.
    Authors: Mohri Y, Mohri T, Wei W, Qi YJ, Martin A, Miki C, Kusunoki M, Ward DG, Johnson PJ
    Br. J. Cancer, 2009-06-23;101(2):295-302.
    Species: Human
    Sample Types: Serum
  26. Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
    Authors: Wang B, Wood IS, Trayhurn P
    J. Endocrinol., 2008-05-07;198(1):127-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007-12-10;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. The role of macrophage migration inhibitory factor in the cascade of events leading to reperfusion-induced inflammatory injury and lethality.
    Authors: Amaral FA, Fagundes CT, Guabiraba R, Vieira AT, Souza AL, Russo RC, Soares MP, Teixeira MM, Souza DG
    Am. J. Pathol., 2007-11-30;171(6):1887-93.
    Species: Mouse
    Sample Types: Serum
  29. Panel of markers can accurately predict endometriosis in a subset of patients.
    Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
    Fertil. Steril., 2007-08-13;89(5):1073-81.
    Species: Human
    Sample Types: Serum
  30. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
    Authors: Schubert T, Horch RE
    Ann. Surg., 2004-08-01;240(2):340-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  32. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
    Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
    Endocrinology, 2004-06-10;145(9):4084-93.
    Species: Rat
    Sample Types: Tissue Homogenates

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Human MIF Quantikine ELISA Kit
By Anonymous on 06/18/2022
Sample Tested: 293T human embryonic kidney cell line
Human MIF Quantikine ELISA Kit DMF00B
Human MIF Quantikine ELISA Kit
By Anonymous on 05/26/2020
Sample Tested: EDTA Plasma
Human MIF Quantikine ELISA Kit DMF00B