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Human Mer PE-conjugated Antibody

Catalog #: FAB8912P
11 Citations Datasheet
Catalog # Availability Size / Price Qty
FAB8912P
Detection of Mer in HepG2 Human hepatocellular Cell Line by Flow Cytometry.
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Citations (11)
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Human Mer PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.
Source
Monoclonal Mouse IgG2B Clone # 125518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Met1-Ala499
Accession # AAB60430
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
Mer is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Mer knockout HepG2 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mer antibody in HepG2 Human hepatocellular Cell Line antibody by Flow Cytometry. View Larger

Detection of Mer in HepG2 Human hepatocellular Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer PE-conjugated Monoclonal Antibody (Catalog # FAB8912P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mer antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Mer in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer PE-conjugated Monoclonal Antibody (Catalog # FAB8912P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Mer Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Mer Specificity is Shown by Flow Cytometry in Knockout Cell Line. Mer knockout HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer PE-conjugated Monoclonal Antibody (Catalog # FAB8912P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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Citations for Human Mer PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
    Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
    bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018-01-02;515(0):176-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Axl is required for TGF-?2-induced dormancy of prostate cancer cells in the bone marrow
    Sci Rep, 2016-11-07;6(0):36520.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Coordinated expression of Tryo3, Axl, and Mer receptors in macrophage ontogeny
    Authors: Anna Malawista, Xiaomei Wang, Mark Trentalange, Heather G. Allore, Ruth R. Montgomery
    Macrophage (Houst)
  5. Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta.
    Authors: Dutertre C, Clement M, Morvan M, Schakel K, Castier Y, Alsac J, Michel J, Nicoletti A
    PLoS ONE, 2014-02-27;9(2):e89983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Mer receptor tyrosine kinase is a therapeutic target in pre–B-cell acute lymphoblastic leukemia
    Authors: Rachel M. A. Linger, Alisa B. Lee-Sherick, Deborah DeRyckere, Rebecca A. Cohen, Kristen M. Jacobsen, Amy McGranahan et al.
    Blood
  7. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
    Authors: Zizzo G, Cohen P
    J Immunol, 2013-04-17;190(10):5237-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
    Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
    Arthritis Res Ther, 2013-01-01;15(6):R212.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Using Flow Cytometry to Compare the Dynamics of Photoreceptor Outer Segment Phagocytosis in iPS-Derived RPE Cells
    Authors: Peter D. Westenskow, Stacey K. Moreno, Tim U. Krohne, Toshihide Kurihara, Saiyong Zhu, Zhen-ning Zhang et al.
    Investigative Opthalmology & Visual Science
  10. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Ectopic expression of the proto-oncogene Mer in pediatric T-cell acute lymphoblastic leukemia.
    Authors: Graham DK, Salzberg DB, Kurtzberg J, Sather S, Matsushima GK, Keating AK, Liang X, Lovell MA, Williams SA, Dawson TL, Schell MJ, Anwar AA, Snodgrass HR, Earp HS
    Clin. Cancer Res., 2006-05-01;12(9):2662-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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