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Human Mer Antibody

Catalog #: AF891
20 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF891
AF891-SP
Detection of Human Mer by Western Blot.
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Product Details
Citations (20)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mer Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Mer and less than 1% cross-reactivity with recombinant human (rh) Axl and rhDtk is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Arg26-Ala499
Accession # Q12866
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Mer is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Mer knockout HepG2 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Mer antibody by Western Blot. View Larger

Detection of Human Mer by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human Mer Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Mer Specificity by Using Knockout Cell Line. Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Mer knockout HepG2 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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Citations for Human Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Phosphorylation of Ack1 by the Receptor Tyrosine Kinase Mer
    Authors: Samantha Y. Hayashi, Barbara P. Craddock, W. Todd Miller
    Kinases and Phosphatases
  2. Decoding Functional High-Density Lipoprotein Particle Surfaceome Interactions
    Authors: Frey K, Goetze S, Rohrer L et al.
    International journal of molecular sciences
  3. TIM-1 Mediates Dystroglycan-Independent Entry of Lassa Virus
    Authors: Rachel B. Brouillette, Elisabeth K. Phillips, Radhika Patel, Wadie Mahauad-Fernandez, Sven Moller-Tank, Kai J. Rogers et al.
    Journal of Virology
  4. Antiphospholipid Antibodies Inhibit Trophoblast Toll-Like Receptor and Inflammasome Negative Regulators
    Authors: Melissa J. Mulla, Ingrid C. Weel, Julie A. Potter, Stefan M. Gysler, Jane E. Salmon, Maria T. S. Peraçoli et al.
    Arthritis & Rheumatology
  5. Regulation of Mertk Surface Expression via ADAM17 and ?-Secretase Proteolytic Processing
    Authors: Lahey, KC;Varsanyi, C;Wang, Z;Aquib, A;Gadiyar, V;Rodrigues, AA;Pulica, R;Desind, S;Davra, V;Calianese, DC;Liu, D;Cho, JH;Kotenko, SV;De Lorenzo, MS;Birge, RB;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  6. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
    Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
    Nature Medicine, 2022-08-04;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
    Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
    Cell Death & Disease, 2021-05-25;12(6):538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018-04-03;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018-01-02;515(0):176-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017-10-12;117(12):1787-1797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
    Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
    J. Immunol., 2017-09-15;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Neutralization, Western Blot
  13. AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses
    Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-06;114(8):2024-2029.
    Species: Human, Primate
    Sample Types: Whole Cells
    Applications: Neutralization
  14. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
    Proc Natl Acad Sci USA, 2016-05-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  15. Increased soluble phagocytic receptors sMer, sTyro3 and sAxl and reduced phagocytosis in juvenile-onset systemic lupus erythematosus.
    Authors: Ballantine, Lucy, Midgley, Angela, Harris, David, Richards, Ella, Burgess, Sarah, Beresford, Michael
    Pediatr Rheumatol Online J, 2015-04-10;13(0):10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
    Authors: Zizzo G, Cohen P
    J Immunol, 2013-04-17;190(10):5237-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012-10-15;209(11):2033-47.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  18. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
    Authors: Eken C, Martin PJ, Sadallah S, Treves S, Schaller M, Schifferli JA
    J. Biol. Chem., 2010-10-19;285(51):39914-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
    Authors: Uehara H, Shacter E
    J. Immunol., 2008-02-15;180(4):2522-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Neutralization, Western Blot

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Human Mer Antibody
By Anonymous on 07/03/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUMAN MACROPHAGES Species: Human