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Human Magnetic Luminex® Performance Assay Base Kit, High Sensitivity Cytokine A

Catalog #: LHSCM000
18 Citations 1 Review Datasheet
Luminex Performance Assay for High Sensitivity detection of up to 12 human cytokines

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Human Magnetic Luminex Performance Assay Base Kit, High Sensitivity Cytokine A
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Summary Configure Your Assay Product Features Precision & Accuracy Data Examples Product Datasheets

Human Magnetic Luminex® Performance Assay Base Kit, High Sensitivity Cytokine A Summary

Panel Luminex Performance Human High Sensitivity Cytokine Panel A
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Magnetic beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 20 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across three different concentrations of analytes across 20 different serum assays.
  n=20 (10x2 levels) n=20
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
GM-CSF 4.2 13.3
IFN-gamma 4.5 12.2
IL-1 beta 5.2 12.8
IL-2 5.7 9.8
IL-4 5.3 10.3
IL-5 4.5 11.7
IL-6 4.9 9.7
IL-8 7.1 11.6
IL-10 5.9 10.9
IL-12 5.0 11.1
TNF-alpha 4.2 9.8
VEGF 7.0 13.4
 

Accuracy

Linearity - The data represents mean spiked linearity in serum matrix samples.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
  Linearity Spiked
Recovery
Analyte N Average % N Average %
GM-SCF 4 100 4 102
IFN-gamma 4 100 4 105
IL-1 beta 4 113 4 100
IL-2 4 104 4 96
IL-4 4 104 4 110
IL-5 4 116 4 85
IL-6 4 113 4 100
IL-8 4 123 4 86
IL-10 4 101 4 107
IL-12 4 100 4 103
TNF-alpha 4 112 4 86
VEGF 4 121 4 84
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human Magnetic Luminex® Performance Assay Base Kit, High Sensitivity Cytokine A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Correlation between Thyroid Responses and Inflammatory Cytokines in Critically Ill COVID-19 Patients
    Authors: A Figueras C, MA Ballestero, A Iglesias C, A Barceló, JA Barea-Mend, P Argente De, B Guardiola, J Pérez-Bárc, JA Llompart-P
    Biomedicines, 2022-12-22;11(1):.
    Species: Human
    Sample Types: Serum
  2. Interleukin-8 and depressive responses to an inflammatory challenge: secondary analysis of a randomized controlled trial
    Authors: JL Kruse, CC Boyle, R Olmstead, EC Breen, SJ Tye, NI Eisenberge, MR Irwin
    Scientific Reports, 2022-07-24;12(1):12627.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. IL-2, IL-6 and chitinase 3-like 2 might predict early relapse activity in multiple sclerosis
    Authors: M Petržalka, E Meluzínová, J Libertínov, H Mojžišová, J Hanzalová, P Ro?ková, M Elišák, S Kmetonyová, J Šanda, O Sobek, P Marusi?
    PLoS ONE, 2022-06-27;17(6):e0270607.
    Species: Human
    Sample Types: Serum
  4. Acute aerobic exercise reveals FAHFAs distinguish the metabolomes of overweight and normal weight runners
    Authors: AB Nelson, LS Chow, DB Stagg, JR Gillingham, MD Evans, M Pan, CC Hughey, CL Myers, X Han, PA Crawford, P Puchalska
    JCI Insight, 2022-04-08;0(0):.
    Species: Human
    Sample Types: Serum
  5. Systemic inflammation and sympathetic activation in gestational diabetes mellitus with obstructive sleep apnea
    Authors: O Serednytsk, A Alonso-Fer, C Ribot, A Herranz, A Álvarez, A Sánchez, P Rodríguez, AV Gil, C Pía, JP Cubero, M Barceló, M Cerdà, M Codina, M D Peña, A Barceló, A Iglesias, D Morell-Gar, JA Peña, MP Giménez, MC Piñas, F García-Río
    BMC pulmonary medicine, 2022-03-18;22(1):94.
    Species: Human
    Sample Types: Plasma
  6. Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus
    Authors: VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo
    Viruses, 2021-09-08;13(9):.
    Species: Human
    Sample Types: Plasma
  7. SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response
    Authors: J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Lindstrom-, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton
    Cell Stem Cell, 2020-09-18;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response
    Authors: J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton
    bioRxiv, 2020-08-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB?EGF
    Authors: E Fu, T Liu, S Yu, X Chen, L Song, H Lou, F Ma, S Zhang, S Hussain, J Guo, J Sun, P Yu, X Mao, L Wei
    Oncol. Rep., 2020-05-29;44(2):698-710.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Transforming growth factor-beta profiles correlate with clinical symptoms and parameters of haemostasis and inflammation in a controlled human malaria infection
    Authors: GM de Jong, MBB McCall, WA Dik, RT Urbanus, LJ Wammes, R Koelewijn, RW Sauerwein, A Verbon, JJ van Hellem, PJJ van Gender
    Cytokine, 2019-09-13;125(0):154838.
    Species: Human
    Sample Types: Serum
  11. Elevated numbers of PD-L1 expressing B cells are associated with the development of AIDS-NHL
    Authors: M Epeldegui, DV Conti, Y Guo, W Cozen, ML Penichet, O Martínez-M
    Sci Rep, 2019-06-28;9(1):9371.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Patients with idiopathic recurrent miscarriage have abnormally high TGF�+ blood NK, NKT and T cells in the presence of abnormally low TGF� plasma levels
    Authors: L Zhu, M Aly, RJ Kuon, B Toth, H Wang, H Karakizlis, R Weimer, C Morath, E Ibrahim, N Ekpoom, G Opelz, V Daniel
    BMC Immunol., 2019-03-04;20(1):10.
    Species: Human
    Sample Types: Plasma
  13. Moderators for depressed mood and systemic and transcriptional inflammatory responses: a randomized controlled trial of endotoxin
    Authors: MR Irwin, S Cole, R Olmstead, EC Breen, JJ Cho, M Moieni, NI Eisenberge
    Neuropsychopharmacology, 2018-11-03;44(3):635-641.
    Species: Human
    Sample Types: Plasma
  14. ?-3 fatty acids-supplementary in gestation alleviates neuroinflammation and modulates neurochemistry in rats
    Authors: M Tang, R Dang, S Liu, M Zhang, Y Zheng, R Yang, T Yin
    Lipids Health Dis, 2018-11-03;17(1):247.
    Species: Rat
    Sample Types: Serum
  15. Short-term cactus pear [Opuntia ficus-indica (L.) Mill] fruit supplementation ameliorates the inflammatory profile and is associated with improved antioxidant status among healthy humans
    Authors: A Attanzio, L Tesoriere, S Vasto, AM Pintaudi, MA Livrea, M Allegra
    Food Nutr Res, 2018-08-20;62(0):.
    Species: Human
    Sample Types: Plasma
  16. The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA
    Authors: AM Quinn, AR Williams, TI Sivilli, CL Raison, TWW Pace
    Stress, 2018-03-13;0(0):1-6.
    Species: Human
    Sample Types: Plasma
  17. Cytokines and Postoperative Delirium in Older Patients Undergoing Major Elective Surgery.
    Authors: Vasunilashorn S, Ngo L, Inouye S, Libermann T, Jones R, Alsop D, Guess J, Jastrzebski S, McElhaney J, Kuchel G, Marcantonio E
    J Gerontol A Biol Sci Med Sci, 2015-07-27;0(0):.
    Species: Human
    Sample Types: Plasma
  18. Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma.
    Authors: Beane J, Lee G, Zheng Z, Mendel M, Abate-Daga D, Bharathan M, Black M, Gandhi N, Yu Z, Chandran S, Giedlin M, Ando D, Miller J, Paschon D, Guschin D, Rebar E, Reik A, Holmes M, Gregory P, Restifo N, Rosenberg S, Morgan R, Feldman S
    Mol Ther, 2015-05-05;23(8):1380-90.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human Mag Luminex Performance Assay Base Kit, HS Cytokine A
By Anonymous on 10/19/2019
Sample Tested: in vitro cell culture