Human M-CSF PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of M‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS and Recombinant Human TNF-a (210-TA) were stained with Mouse Anti-Human M-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Detection of M‑CSF in MG-63 cells by Flow Cytometry. MG-63 cells were stained with Mouse Anti-Human M‑CSF PE‑conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: M-CSF
M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.
Product Datasheets
Citation for Human M-CSF PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome
Authors: M Tkach, J Thalmensi, E Timperi, P Gueguen, N Névo, E Grisard, P Sirven, F Cocozza, A Gouronnec, L Martin-Jau, M Jouve, F Delisle, N Manel, DC Rookhuizen, CL Guerin, V Soumelis, E Romano, E Segura, C Théry
Proceedings of the National Academy of Sciences of the United States of America, 2022-04-19;119(17):e2107394119.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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