Human M-CSF Antibody Summary
Glu33-Ser190
Accession # NP_757350
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody. Recombinant Human M-CSF (Catalog # 216-MC) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M-CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216). The ND50 is typically 0.01-0.03 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-CSF
M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
Product Datasheets
Citations for Human M-CSF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Fucoidan modulates cytokine production and migration of THP-1-derived macrophages via colony-stimulating factor-1
Authors: Peng Li, Huayang Wang, Qianqian Shao, Beihua Kong, Xun Qu
Molecular Medicine Reports
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Crosstalk between macrophage-derived PGE2 and tumor UHRF1 drives hepatocellular carcinoma progression
Authors: J Zhang, H Zhang, X Ding, J Hu, Y Li, J Zhang, H Wang, S Qi, A Xie, J Shi, M Xiang, Y Bin, G Wang, L Wang, Z Wang
Theranostics, 2022-05-01;12(8):3776-3793.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot -
Long non-coding RNA HOMER3-AS1 drives hepatocellular carcinoma progression via modulating the behaviors of both tumor cells and macrophages
Authors: J Pu, W Li, A Wang, Y Zhang, Z Qin, Z Xu, J Wang, Y Lu, Q Tang, H Wei
Cell Death & Disease, 2021-11-23;12(12):1103.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
Redox Biol, 2019-02-06;22(0):101116.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis.
Authors: Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR
Blood, 2008-12-05;113(9):1992-2002.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1
Authors: Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang, Chang-li Wang
Cellular & Molecular Biology Letters
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