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Human Livin Antibody

Catalog #: AF1161
2 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1161
AF1161-SP
Detection of Human Livin by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Livin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Livin in Western blots. Can detect recombinant and endogenous human Livin alpha (KIAP) and Livin beta (ML-IAP).
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Livin beta
Met1-Gln280
Accession # Q96CA5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Livin antibody by Western Blot. View Larger

Detection of Human Livin by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, Recombinant Human Livin a/KIAP (Catalog # 1161-LV) and Recombinant Human Livin beta /ML-IAP (Catalog # 787-LV) (0.5 ng/lane) were included. A specific band for Livin was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Simple Western Detection of Human Livin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Livin by Simple WesternTM. Simple Western lane view shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Livin at approximately 42 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Livin

Livin alpha (KIAP) and Livin beta (ML-IAP) are two splice forms generated from the livin gene. Livin is a member of the inhibitor of apoptosis protein (IAP) family that contains a baculovirus single IAP repeat (BIR) and a C-terminal RING finger domain. Livin inhibits the activation of caspases 9 and/or 3 when activated in specific cell lines. Livin alpha is a splice variant 18 amino acids longer in the BIR-RING linking region than the livin beta splice variant. The isoforms differ in their apoptotic properties.

Entrez Gene IDs
79444 (Human)
Alternate Names
baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; BIRC7; KIAP; KIAPRING finger protein 50; Kidney inhibitor of apoptosis protein; livin inhibitor-of-apoptosis; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAPlivin inhibitor of apoptosis; ML-IAPmliap; RNF50LIVINbaculoviral IAP repeat-containing protein 7

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Citations for Human Livin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
    Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
    PLoS ONE, 2012-09-20;7(9):e45492.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
    Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
    J Invest Dermatol, 2011-06-30;131(11):2327-9.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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