Human Lipocalin-2/NGAL Quantikine QuicKit ELISA

Catalog #: QK1757 Datasheet

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QK1757

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QC286: Human Lipocalin-2/NGAL Quantikine QuicKit Control Group 286

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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Lipocalin-2/NGAL Quantikine QuicKit ELISA Summary

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL)

Sensitivity

0.033 ng/mL

Assay Range

0.3 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human Lipocalin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Sample Type	Mean (ng/mL)	Range (ng/mL)	Standard Deviation (ng/mL)
Serum (n=10)	79.3	50.9-129	25.7
Heparin Plasma (n=10)	64.3	46.3-94.9	18.3
Saliva (n=10)	387	62.1-734	217
Urine (n=10)	11.3	1.18-27.9	9.37

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human Lipocalin-2 in this assay. No medical histories were available for the donors used in this study.

Cell Culture Supernates

Condition	Day 1(ng/mL)	Day 5 (ng/mL)
Unstmulated	2.59	5.18
Stimulated	3.28	4.58

Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human Lipocalin-2.

Product Summary

The Quantikine™ QuicKit™ Human Lipocalin-2/NGAL Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Lipocalin-2 levels in cell culture supernates, serum, heparin plasma, saliva, and urine. It contains NS0-expressed recombinant human Lipocalin-2 and antibodies raised against the recombinant protein. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human Lipocalin-2.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (ng/mL)	1.01	6.05	1.11	6.46
Standard Deviation	0.025	0.261	0.058	0.379
CV%	2.5	4.3	5.2	5.9

Recovery

The recovery of human Lipocalin-2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=)
Urine (n=2)	102	89-110

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Lipocalin-2 in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human Lipocalin-2 Standard Curve ELISA

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:

Neutrophil Gelatinase-associated Lipocalin

Entrez Gene IDs:

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Alternate Names:

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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