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Human Lipin 1 Antibody

Catalog #: AF3885
5 Citations Datasheet
Catalog # Availability Size / Price Qty
AF3885
AF3885-SP
Lipin 1 in Human Adipocytes.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lipin 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lipin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Lipin 1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Lipin 1 antibody in Human Adipocytes by Immunocytochemistry (ICC). View Larger

Lipin 1 in Human Adipocytes. Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 1

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.

Long Name
Phosphatidate Phosphatase LPIN1
Entrez Gene IDs
23175 (Human); 14245 (Mouse); 313977 (Rat)
Alternate Names
DKFZp781P1796; EC 3.1.3.18; EC 3.1.3.4; EC 5.4.2.7; KIAA0188phosphatidate phosphatase LPIN1; Lipin 1; lipin-1; LPIN1; PAP1

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Citations for Human Lipin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes
    Authors: M Chae, ED Son, IH Bae, EG Cho, HJ Kim, JY Jung
    Exp. Mol. Med., 2020-02-21;0(0):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver
    Authors: T Arai, M Tanaka, N Goda
    Sci Rep, 2018-09-21;8(1):14230.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP, Western Blot
  3. Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase
    Authors: K Ishimoto, A Hayase, F Kumagai, M Kawai, H Okuno, N Hino, Y Okada, T Kawamura, T Tanaka, T Hamakubo, J Sakai, T Kodama, K Tachibana, T Doi
    Biochem. Biophys. Res. Commun., 2017-05-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein e-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway.
    Authors: Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, Arata S, Yamaguchi T, Watanabe M, Itabe H
    PLoS ONE, 2011-08-09;6(8):e22917.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  5. Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells.
    Authors: Ishimoto K, Nakamura H, Tachibana K, Yamasaki D, Ota A, Hirano K, Tanaka T, Hamakubo T, Sakai J, Kodama T, Doi T
    J. Biol. Chem., 2009-06-24;284(33):22195-205.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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