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Human LAP (TGF-beta 1) Antibody

Catalog #: MAB246
13 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB246-SP
MAB246-500
MAB246-100
LAP (TGF-beta 1) in HEK293 Human Cell Line.
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Citations (13)
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Human LAP (TGF-beta 1) Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 27235
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL Recombinant Human TGF‑ beta 1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry LAP (TGF-beta 1) antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

LAP (TGF-beta 1) in HEK293 Human Cell Line. LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. View Larger

LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70-80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF-beta 1, TGF‑ beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name
Latency-associated Peptide
Entrez Gene IDs
7040 (Human)
Alternate Names
CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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Citations for Human LAP (TGF-beta 1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
    Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
    Front Immunol, 2022-09-29;13(0):933329.
    Species: Complex Species Category
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Chemokine Receptor Expression on T Cells Is Modulated by CAFs and Chemokines Affect the Spatial Distribution of T Cells in Pancreatic Tumors
    Authors: L Gorchs, M Oosthoek, T Yucel-Lind, CF Moro, H Kaipe
    Cancers, 2022-08-06;14(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Mechanical stress determines the configuration of TGF&beta activation in articular cartilage
    Authors: G Zhen, Q Guo, Y Li, C Wu, S Zhu, R Wang, XE Guo, BC Kim, J Huang, Y Hu, Y Dan, M Wan, T Ha, S An, X Cao
    Nature Communications, 2021-03-17;12(1):1706.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Macrophage-derived HIV-1 carries bioactive TGF-beta
    Authors: A Arakelyan, JD Petersen, J Blazkova, L Margolis
    Sci Rep, 2019-12-13;9(1):19100.
    Species: Virus - HIV-1
    Sample Types: Virus
    Applications: Multiplex Assay
  6. Human Pancreatic Carcinoma-Associated Fibroblasts Promote Expression of Co-inhibitory Markers on CD4+ and CD8+ T-Cells
    Authors: Laia Gorchs, Carlos Fernández Moro, Peter Bankhead, Katharina P. Kern, Imrul Sadeak, Qingda Meng et al.
    Frontiers in Immunology
  7. Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC
    Authors: H Sun, J Xu, Q Huang, M Huang, K Li, K Qu, H Wen, R Lin, M Zheng, H Wei, W Xiao, R Sun, Z Tian, C Sun
    Cancer Res., 2018-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus
    Authors: YD Boakye, C Agyare, GP Ayande, N Titiloye, EA Asiamah, KO Danquah
    Front Pharmacol, 2018-08-21;9(0):945.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Lesional CD4+ IFN-gamma + cytotoxic T lymphocytes in IgG4-related dacryoadenitis and sialoadenitis
    Authors: Takashi Maehara, Hamid Mattoo, Miho Ohta, Vinay S Mahajan, Masafumi Moriyama, Masaki Yamauchi et al.
    Annals of the Rheumatic Diseases
  10. LAP degradation product reflects plasma kallikrein-dependent TGF-beta activation in patients with hepatic fibrosis
    Authors: Mitsuko Hara, Akiko Kirita, Wakako Kondo, Tomokazu Matsuura, Keisuke Nagatsuma, Naoshi Dohmae et al.
    SpringerPlus
  11. Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner.
    Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S
    Immunology, 2011-03-23;133(3):278-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004-12-01;34(12):3485-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization

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