Human LAP (TGF-beta 1) Antibody

Catalog #: MAB7754 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7754-100
MAB7754-SP

Human LAP (TGF‑ beta 1) ELISA Standard Curve.

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Human LAP (TGF-beta 1) Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAP (TGF‑ beta 1) in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1015016

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LAP (TGF‑ beta 1)
Met1-Ser390
Accession # P01137

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LAP (TGF-beta 1) DuoSet ELISA Kit (Catalog # DY246) for convenient development of a sandwich ELISA or the Human LAP (TGF-beta 1) Quantikine ELISA Kit (Catalog # DLAP00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human LAP (TGF‑ beta 1) ELISA Standard Curve. Recombinant Human LAP (TGF-beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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