Human Klotho DuoSet ELISA

Catalog #: DY5334-05
12 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
DY5334-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

1 Image

All Klotho ELISAs

Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Klotho DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

78.1 - 5,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Klotho. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

View Larger

Human Klotho ELISA Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Klotho

The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging.

Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.

Entrez Gene IDs:

9365 (Human); 16591 (Mouse)

Alternate Names:

EC 3.2.1; EC 3.2.1.31; KL; Klotho

Citations for Human Klotho DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Filter by:

Serum Klotho Is Elevated in Patients with Acute Myocardial Infarction and Could Predict Poor In-Hospital Prognosis
Authors: Pei, Y;Huang, W;Cao, L;Yang, F;Chi, C;Zhu, J;
Journal of cardiovascular development and disease
Species: Human
Sample Types: Serum
The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients
Authors: Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E;
International journal of molecular sciences
Species: Human
Sample Types: Serum
Effects of low-dose ketamine infusion on vascular endothelial growth factor and matrix metalloproteinase-9 among patients with treatment-resistant depression and suicidal ideation
Authors: Chen, MH;Lin, WC;Li, CT;Wu, HJ;Bai, YM;Tsai, SJ;Su, TP;Tu, PC;
Journal of psychiatric research
Species: Human
Sample Types: Serum
The Circulating Level of Klotho Is Not Dependent upon Physical Fitness and Age-Associated Methylation Increases at the Promoter Region of the Klotho Gene
Authors: D Aczel, F Torma, M Jokai, K McGreevy, A Boros, Y Seki, I Boldogh, S Horvath, Z Radak
Genes, 2023-02-19;14(2):.
Species: Human
Sample Types: Plasma
Association of serum klotho levels with different-staged vascular calcification status in patients with maintenance hemodialysis
Authors: Y Lin, J Huang, M Zhou, C Pan, A Shan, C Zhou
Bmc Nephrology, 2022-11-19;23(1):374.
Species: Human
Sample Types: Serum
Combined resistance and aerobic training improves lung function and mechanics and fibrotic biomarkers in overweight and obese women
Authors: A Silva-Reis, MA Rodrigues, R Moraes-Fer, TG Gonçalves-, VH Souza-Palm, HC Aquino-San, ALL Bachi, LVF de Oliveir, RÁB Lopes-Mart, I Oliveira-S, R Albertini, CR Frison, RP Vieira
Frontiers in Physiology, 2022-09-07;13(0):946402.
Species: Human
Sample Types: Plasma
Klotho and SIRT1 changes from pre-diabetes to diabetes and pre-hypertension to hypertension
Authors: M Yeganeh-Ha, H Najafipour, F Rostamzade, A Naghibzade
Diabetology & metabolic syndrome, 2021-10-20;13(1):115.
Species: Human
Sample Types: Serum
Pulmonary function changes in older adults with and without metabolic syndrome
Authors: MAR Brandao-Ra, R Moraes-Fer, MC Oliveira-J, A Santos-Dia, ALL Bachi, G Gabriela-P, S de Oliveir, AC Araújo-Ros, LVF Oliveira, CR Frison, WL do Prado, RP Raju, PB Balagopal, RP Vieira
Scientific Reports, 2021-08-30;11(1):17337.
Species: Human
Sample Types: Exhaled Breath Condensate (EBC)
No significant association of serum klotho concentration with blood pressure and pulse wave velocity in a Chinese population
Authors: WY Liang, LH Wang, JH Wei, QL Li, QY Li, Q Liang, NQ Hu, LH Li
Scientific Reports, 2021-01-27;11(1):2374.
Species: Human
Sample Types: Serum
Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy
Authors: A Farías-Bas, HR Martínez-R, EF Gómez-Garc, AM Cueto-Manz, L Cortés-San, LE Hernández-, G Ramírez-Ló, F Mendoza-Ca
Arch. Med. Res., 2019-02-01;0(0):.
Species: Human
Sample Types: Serum

Associations of sclerostin with carotid artery atherosclerosis and all-cause mortality in Chinese patients undergoing maintenance hemodialysis
Authors: A Chen, Y Sun, J Cui, B Zhao, H Wang, X Chen, Y Mao
BMC Nephrol, 2018-10-12;19(1):264.
Species: Human
Sample Types: Plasma

Sclerostin, TNF-alpha and Interleukin-18 Correlate and are Together with Klotho Related to Other Growth Factors and Cytokines in Haemodialysis Patients.
Authors: Almroth G, Lonn J, Uhlin F, Brudin L, Andersson B, Hahn-Zoric M
Scand J Immunol, 2016-01-01;83(1):58-63.
Species: Human
Sample Types: Serum